Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, Heilongjiang, China.
Antiviral Res. 2010 Feb;85(2):422-4. doi: 10.1016/j.antiviral.2009.10.010. Epub 2009 Oct 24.
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious fatal disease of swine. Few effective antiviral drugs are currently available against CSFV infections. To explore the feasibility of using capsid-targeted viral inactivation (CTVI) as an antiviral strategy against CSFV infections, we expressed the CSFV capsid protein (Cap) fused with the nuclease of Staphylococcus aureus (SN) in Escherichia coli and investigated its effects on the replication of CSFV in PK-15 cells. The results indicated that the fusion protein Cap-SN showed a strong Ca(2+)-dependent nuclease activity and inhibited the replication of CSFV in a dose-dependent manner, with complete inhibition at a concentration of 15 microg/ml, whereas the Cap fused with an enzymatically inactive SN (Cap-SN*) showed no nuclease activity or antiviral effects. Thus, the CTVI approach might be applicable to CSFV inhibition as a novel antiviral strategy.
经典猪瘟病毒（CSFV）是经典猪瘟（CSF）的病原体，CSF 是一种高度传染性的致命猪病。目前针对 CSFV 感染，有效的抗病毒药物寥寥无几。为了探索基于衣壳蛋白的病毒失活（CTVI）作为一种抗 CSFV 感染的抗病毒策略的可行性，我们在大肠杆菌中表达了与金黄色葡萄球菌核酸酶融合的 CSFV 衣壳蛋白（Cap），并研究了其对 PK-15 细胞中 CSFV 复制的影响。结果表明，融合蛋白 Cap-SN 表现出强烈的 Ca2+依赖性核酸酶活性，并以剂量依赖性方式抑制 CSFV 的复制，在 15 μg/ml 的浓度下完全抑制，而与无酶活性的 SN 融合的 Cap（Cap-SN*）则没有核酸酶活性或抗病毒作用。因此，CTVI 方法可能适用于 CSFV 的抑制，作为一种新的抗病毒策略。
