Department of Neurobiology and Anatomy, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, Jiangsu, 221002, China.
Neurosci Lett. 2010 Jan 1;468(1):3-6. doi: 10.1016/j.neulet.2009.10.044. Epub 2009 Oct 24.
We studied the relationship between aging and the vulnerability of substantia nigra pars compacta (SNc) calbindin-D-28k immunoreactive positive (CB+) dopaminergic (DA) neurons. Immunohistochemistry and cell counting were used to determine the number of CB+ DA neuron in aged rats (24 mon) compared to adult rats (5 mon). Furthermore, the expression of CB mRNA and protein levels in SN was studied by semi-quantitative RT-PCR and Western blotting. An 11% loss of CB+ DA neurons was detected in both the rostral (8.9%) and caudal (1.7%) segments but not in the intermedial segment of SNc in aged rats compared to adult rats (P<0.05). No difference was detected in CB mRNA and protein levels between aged and adult rats (P>0.05). These data suggest that expression levels of CB mRNA and protein may increase in the existing SNc DA neurons, which may compensate for the partial age dependent loss of CB+ DA neurons in the SNc.
我们研究了衰老与黑质致密部（SNc）钙结合蛋白-D-28k 免疫反应阳性（CB+）多巴胺能（DA）神经元易损性之间的关系。免疫组织化学和细胞计数用于确定与成年大鼠（5 月龄）相比，老年大鼠（24 月龄）中 CB+DA 神经元的数量。此外，通过半定量 RT-PCR 和 Western 印迹研究了 SN 中 CB mRNA 和蛋白水平的表达。与成年大鼠相比，老年大鼠的 SNc 头段（8.9%）和尾段（1.7%）但中段（1.7%）的 CB+DA 神经元丢失了 11%（P<0.05）。在 CB mRNA 和蛋白水平上，老年大鼠与成年大鼠之间没有差异（P>0.05）。这些数据表明，CB mRNA 和蛋白的表达水平可能在现有的 SNc DA 神经元中增加，这可能补偿了 SNc 中 CB+DA 神经元的部分年龄依赖性丢失。
