Miao Yinglei, Li Wenliang, Duan Liping, Xiao Yuliang
Department of Gastroenterology, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China.
Comp Biochem Physiol B Biochem Mol Biol. 2010 Feb;155(2):106-9. doi: 10.1016/j.cbpb.2009.10.007.
Bombesin-like peptides (BLPs) are a family of neuro-endocrinic peptides that mediates a variety of biological activities. A BLP named bombesin-SV from the skin secretions of the frog, Sanguirana varians, was purified and characterized. Its amino acid sequence is pEMIFGAPMWALGHLM-NH2 determined by Edman degradation and mass spectrometry. The cDNA encoding bombesin-SV precursor was cloned from skin cDNA library of S. varians. The precursor is composed of 67 amino acid residues including a copy of mature bombesin-SV. Two predicted enzymatic processing sites (-Lys-Arg-) are flanked at the mature bombesin-SV sequence. In the in vitro myotropic contraction experiment, the synthesized bombesin-SV displayed the stimulating effect toward rat stomach strips, suggesting that bombesin-SV acts as agonist as most other BLPs do.
蛙皮素样肽（BLPs）是一类介导多种生物活性的神经内分泌肽。从棘胸蛙皮肤分泌物中纯化并鉴定出一种名为蛙皮素-SV的BLP。通过埃德曼降解法和质谱法确定其氨基酸序列为pEMIFGAPMWALGHLM-NH2。从棘胸蛙皮肤cDNA文库中克隆出编码蛙皮素-SV前体的cDNA。该前体由67个氨基酸残基组成，包括一个成熟蛙皮素-SV拷贝。在成熟蛙皮素-SV序列两侧有两个预测的酶切位点（-Lys-Arg-）。在体外肌动收缩实验中，合成的蛙皮素-SV对大鼠胃条显示出刺激作用，表明蛙皮素-SV与大多数其他BLPs一样作为激动剂发挥作用。
