Department of Surgery, Division of Cardiothoracic Surgery, Columbia University College of Physicians and Surgeons, New York, NY, USA.
J Cardiovasc Pharmacol. 2010 Jan;55(1):14-20. doi: 10.1097/FJC.0b013e3181c5e743.
The effects of exogenous B-type natriuretic peptide (BNP) on postmyocardial infarction (MI) are not known. We tested the hypothesis that in vivo infusion of BNP would improve cardiac function and affect left ventricular (LV) remodeling in an experimental model of MI.
MI was induced by coronary ligation in rats and confirmed by echocardiography. 19 rats were randomized to 1 of 3 groups: sham (n = 7), MI + saline (n = 5), MI + BNP (400 ng.kg(-1).minute(-1)) (n = 7). Infusions were delivered for 7 days via venous catheters tunneled to an infusion pump. Rats were followed for 8 weeks. Echocardiography, hemodynamics, histology, and in vivo and ex vivo pressure-volume relationships were examined.
LV systolic pressure, LV dP/dtmax, and infarct size improved with BNP treatment versus control MI group (132 +/- 4 vs.110 +/- 2 mm Hg, 8097 +/- 317 vs. 5816 +/- 378 mm Hg/s, 19.3% +/- 1.6% vs. 23.3% +/- 1.9%, respectively; all P < 0.05). Ex vivo end-diastolic pressure-volume relationship demonstrated reduced diastolic dysfunction after BNP therapy (P < 0.05 vs. control MI). Serum BNP levels confirmed delivery of BNP.
We demonstrate beneficial effects on LV function and decreased LV remodeling with BNP infusion in an experimental model of acute MI.
外源性 B 型利钠肽（BNP）对心肌梗死后（MI）的影响尚不清楚。我们假设在 MI 的实验模型中，BNP 的体内输注将改善心功能并影响左心室（LV）重塑。
通过冠状动脉结扎在大鼠中诱导 MI，并通过超声心动图确认。19 只大鼠随机分为 3 组之一：假手术（n = 7）、MI +盐水（n = 5）、MI + BNP（400ng·kg-1·min-1）（n = 7）。通过静脉导管输注，将导管经隧道连接至输注泵，输注持续 7 天。大鼠随访 8 周。检查超声心动图、血流动力学、组织学以及体内和体外压力-容积关系。
与对照 MI 组相比，BNP 治疗后 LV 收缩压、LV dp/dtmax 和梗死面积改善（132 +/- 4 对 110 +/- 2mmHg，8097 +/- 317 对 5816 +/- 378mmHg/s，19.3% +/- 1.6% 对 23.3% +/- 1.9%；均 P < 0.05）。体外舒张末期压力-容积关系显示 BNP 治疗后舒张功能障碍减轻（P < 0.05 对对照 MI）。血清 BNP 水平证实了 BNP 的输送。
我们在急性 MI 的实验模型中证明了 BNP 输注对 LV 功能的有益影响和 LV 重塑的减少。
