Lee Michael Robert, Pawlowski Karen Sue, Luong Amber, Furze Alexis Dorian, Roland Peter Sargent
Department of Otolaryngology, Head and Neck Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390-9035, USA.
Otolaryngol Head Neck Surg. 2009 Nov;141(5):567-71. doi: 10.1016/j.otohns.2009.08.010.
To study the presence of biofilm formation in humans with chronic suppurative otitis media (CSOM).
Cross-sectional study.
Tertiary academic hospital.
Patients undergoing middle ear surgery between July 2006 and March 2008. Tissue samples were obtained from 25 patients, of which 20 specimens were successfully processed for this study. The remaining samples were not analyzed due to sample damage or loss during preparation. Of the 20 specimens studied, 10 were harvested as the experimental group from patients with CSOM and the other 10 harvested as controls from patients undergoing otologic surgery for acoustic neuroma, cochlear implant, or routine tympanoplasty. Ages ranged from 26 to 74 years (mean 45 yrs). Male-to-female ratio was 2:3. Scanning electron microscopy and confocal laser scanning microscopy were used to identify the presence of biofilms. Live-dead staining was used to assess whether bacteria present were viable. The outcome measured was the presence of adherent biofilms on middle ear mucosa.
Biofilms were present in six of 10 samples (60%) from the CSOM group, but only in one of 10 control samples (10%). Comparative analysis revealed a statistically significant difference (P < 0.05) in the presence of biofilms in specimens from the CSOM group versus the control group.
Biofilms were statistically more common in patients with CSOM compared with control patients.
研究慢性化脓性中耳炎（CSOM）患者中耳生物膜的形成情况。
横断面研究。
三级学术医院。
选取2006年7月至2008年3月期间接受中耳手术的患者。从25例患者获取组织样本，其中20份标本成功用于本研究。其余样本因制备过程中样本损坏或丢失未进行分析。在研究的20份标本中，10份取自CSOM患者作为实验组，另外10份取自因听神经瘤、人工耳蜗植入或常规鼓室成形术接受耳科手术的患者作为对照组。年龄范围为26至74岁（平均45岁）。男女比例为2:3。采用扫描电子显微镜和共聚焦激光扫描显微镜鉴定生物膜的存在。采用活死染色法评估存在的细菌是否存活。测量的结果是中耳黏膜上附着生物膜的存在情况。
CSOM组10份样本中有6份（60%）存在生物膜，而对照组10份样本中仅有1份（10%）存在生物膜。比较分析显示，CSOM组标本与对照组标本中生物膜的存在情况存在统计学显著差异（P < 0.05）。
与对照组患者相比，CSOM患者中耳生物膜在统计学上更为常见。
