[皮下植入胚胎皮肤细胞构建裸鼠毛发发育模型]
[Subcutaneous implantation of embryonic skin cells to construct a model of hair development in nude mice].
作者信息
Chen Ming-juan, Hu Zhi-qi, Tan Ting, Sun Xi-jin, Miao Yong
机构信息
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. doctor.cmj13520 @yahoo.com.cn
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Oct;29(10):2024-6.
OBJECTIVE
To observe the hair development after subcutaneous implantation of embryonic skin cells in nude mice, and construct a model of hair development.
METHODS
Dermal and epidermal cells isolated from embryonic mouse skin were mixed at a given ratio and injected subcutaneously in nude mice, and hair formation after the implantation was observed under a microscope.
RESULTS
Formation of the hair follicles and fibers under the skin of the recipient nude mice and emergence of the hair shaft were observed microscopically.
CONCLUSION
Embryonic skin cells be used to construct a complete model of hair development after implantation in vivo.
目的
观察胚胎皮肤细胞皮下植入裸鼠后的毛发发育情况，构建毛发发育模型。
方法
将从胚胎小鼠皮肤分离的真皮和表皮细胞按给定比例混合，皮下注射到裸鼠体内，在显微镜下观察植入后的毛发形成情况。
结果
在受体裸鼠皮肤下观察到毛囊和纤维的形成以及毛干的出现。
结论
胚胎皮肤细胞植入体内后可用于构建完整的毛发发育模型。
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