Racker E
J Supramol Struct. 1977;6(2):215-28. doi: 10.1002/jss.400060207.
Reconstitutions of membranous activities can tell us how many components are required and what their functions are. The mitochondrial proton pump is used as an example. Moreover, the biological activity, such as Pi transport, can be used in reconstituted vesicles as an assay during the isolation of the transporter. Reconstitution experiments reveal the importance of membrane asymmetry and allow us to study conditions of vectorial assembly. The mechanism of action of ion pumps has been successfully analyzed in reconstituted liposomes. We can study the movement of ions and the electrogenicity of the system without interference by other unrelated processes. Based on studies with the resolved Ca2+-ATPase of sarcoplasmic reticulum, we propose a novel formulation of the mechanism of ATP-driven ion pumps in which cyclic binding of Mg2+ plays a key role.
膜活性的重组可以告诉我们需要多少种组分以及它们的功能是什么。以线粒体质子泵为例。此外，诸如磷酸转运等生物活性可在重组囊泡中用作分离转运体过程中的一种测定方法。重组实验揭示了膜不对称性的重要性，并使我们能够研究向量组装的条件。离子泵的作用机制已在重组脂质体中得到成功分析。我们可以研究离子的运动和系统的电生性，而不受其他无关过程的干扰。基于对肌浆网中已解析的Ca2 + -ATP酶的研究，我们提出了一种ATP驱动离子泵机制的新表述，其中Mg2 +的循环结合起关键作用。
