Paediatric Department, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile.
Clin Endocrinol (Oxf). 2010 Apr;72(4):489-95. doi: 10.1111/j.1365-2265.2009.03735.x. Epub 2009 Oct 26.
To assess normative data and the usefulness of spontaneous and LHRH analogue-stimulated serum LH and FSH levels measured by immunoradiometric assays (IRMA) in the evaluation of normal puberty.
Prospective. Healthy girls in Tanner I and Tanner II from the local community were invited to participate (n = 47).
A leuprolide acetate test (500 mcg/m(2); sc) was performed. LH and FSH levels were determined using IRMA. Tanner II girls were assessed every 6 months until Tanner V. Girls who progressed from Tanner II to Tanner III in the next 6 months were called Tanner II-2; otherwise, they were called Tanner II-1.
The prepubertal upper limit (CI 95%) was 0.49 IU/l for basal LH and 5.1 IU/l for stimulated LH. Taking into account these LH cut-off limits, 72.2% and 66.7% of Tanner II-1 and 41.6% and 41.7% of Tanner II-2 subjects presented overlapping values for basal and stimulated LH, respectively, as compared with the Tanner I group. The cut-offs for basal and stimulated LH to predict progression from Tanner II to Tanner III in the next 6 months were a basal LH level > or =0.49 IU/l (Sensitivity = 0.58; 1-Specificity = 0.33) and a poststimulated LH level > or =4.75 IU/l (Sensitivity = 0.67; 1-Specificity = 0.44).
According to an IRMA, the basal and leuprolide acetate gonadotrophin response patterns during the beginning stages of puberty overlapped between Tanner I and Tanner II, and the cut-offs of basal and stimulated LH levels to predict progress from Tanner II to Tanner III had low sensitivities for the following 6 months.
评估通过免疫放射测定法（IRMA）测量的自发和 LHRH 类似物刺激的血清 LH 和 FSH 水平的正常值数据及其在正常青春期评估中的作用。
前瞻性。从当地社区邀请 Tanner I 和 Tanner II 的健康女孩参加（n = 47）。
进行醋酸亮丙瑞林试验（500 mcg/m²；sc）。使用 IRMA 测定 LH 和 FSH 水平。Tanner II 女孩每 6 个月评估一次，直到 Tanner V。在下 6 个月内从 Tanner II 进展到 Tanner III 的女孩称为 Tanner II-2；否则，她们称为 Tanner II-1。
青春期前的上限（95%CI）为基础 LH 的 0.49 IU/l 和刺激 LH 的 5.1 IU/l。考虑到这些 LH 截止值，Tanner II-1 的 72.2%和 66.7%以及 Tanner II-2 的 41.6%和 41.7%的基础和刺激 LH 值与 Tanner I 组重叠。预测下 6 个月从 Tanner II 进展到 Tanner III 的基础和刺激 LH 的截止值为基础 LH 水平>或=0.49 IU/l（灵敏度=0.58；1 特异性=0.33）和刺激后 LH 水平>或=4.75 IU/l（灵敏度=0.67；1 特异性=0.44）。
根据 IRMA，青春期开始时的基础和亮丙瑞林促性腺激素反应模式在 Tanner I 和 Tanner II 之间重叠，预测从 Tanner II 到 Tanner III 进展的基础和刺激 LH 水平的截止值在下 6 个月内的灵敏度较低。
