恒河猴疱疹病毒编码的病毒白细胞介素-6与淋巴增殖性疾病（LPD）相关。
Viral interleukin-6 encoded by rhesus macaque rhadinovirus is associated with lymphoproliferative disorder (LPD).
作者信息
Orzechowska B U, Manoharan M, Sprague J, Estep R D, Axthelm M K, Wong S W
机构信息
Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus, Beaverton, OR 97006, USA.
出版信息
J Med Primatol. 2009 Oct;38 Suppl 1(Suppl 1):2-7. doi: 10.1111/j.1600-0684.2009.00369.x.
BACKGROUND
Rhesus macaques (RM) co-infected with simian immunodeficiency virus (SIV) and rhesus macaque rhadinovirus (RRV) develop abnormal cellular proliferations characterized as extra-nodal lymphoma and retroperitoneal fibromatosis (RF). RRV encodes a viral interleukin-6 (vIL-6), much like Kaposi's sarcoma-associated herpesvirus, and involvement of the viral cytokine was examined in proliferative lesions.
METHODS
Formalin fixed tissue from RM co-infected with SIV and RRV were analyzed for RRV genomes by in situ hybridization and RRV vIL-6 expression by immunofluorescence analysis.
RESULTS
In situ hybridization analysis indicated that RRV is present in both types of lesions. Immunofluorescence analysis of different lymphomas and RF revealed positive staining for vIL-6. Similarly to KS, RF lesion is positive for vimentin, CD117 (c-kit), and smooth muscle actin (SMA) and contains T cell, B cell and monocytes/macrophage infiltrates.
CONCLUSIONS
Our data support the idea that vIL-6 may be critical to the development and progression of lymphoproliferative disorder in RRV/SIV-infected RM.
背景
感染猿猴免疫缺陷病毒（SIV）和恒河猴疱疹病毒（RRV）的恒河猴（RM）会出现异常细胞增殖，表现为结外淋巴瘤和腹膜后纤维瘤病（RF）。RRV编码一种病毒白细胞介素-6（vIL-6），很像卡波西肉瘤相关疱疹病毒，并且研究了病毒细胞因子在增殖性病变中的作用。
方法
通过原位杂交分析感染SIV和RRV的恒河猴经福尔马林固定的组织中的RRV基因组，并通过免疫荧光分析RRV vIL-6的表达。
结果
原位杂交分析表明，两种病变中均存在RRV。对不同淋巴瘤和RF进行免疫荧光分析，结果显示vIL-6呈阳性染色。与卡波西肉瘤类似，RF病变波形蛋白、CD117（c-kit）和平滑肌肌动蛋白（SMA）呈阳性，并且含有T细胞、B细胞以及单核细胞/巨噬细胞浸润。
结论
我们的数据支持这样一种观点，即vIL-6可能对RRV/SIV感染的恒河猴中淋巴增殖性疾病的发生和发展至关重要。
Zotero 插件