一种肌球蛋白重链样多肽在果蝇核孔复合体中的定位。

Berrios M, Fisher P A, Matz E C

Department of Pharmacological Sciences, State University of New York, Stony Brook 11794-8651.

Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):219-23. doi: 10.1073/pnas.88.1.219.

Antibodies previously used for immunofluorescence localization of a myosin heavy chain-like polypeptide to the nuclear envelope in higher eukaryotic cells crossreact with both muscle and nonmuscle isoforms of Drosophila myosin heavy chain. Analyses of Drosophila tissue culture cells and premyogenic embryos suggest that it is the nonmuscle isoform that is associated with the nuclear envelope. Further immunofluorescence and immunoelectron microscopy indicate that this polypeptide is associated with nuclear pore complexes. These data support the hypothesis put forward previously that myosin or myosin-like molecules play a role in pore complex architecture.

先前用于在高等真核细胞中将一种肌球蛋白重链样多肽免疫荧光定位至核膜的抗体，可与果蝇肌球蛋白重链的肌肉型和非肌肉型亚型发生交叉反应。对果蝇组织培养细胞和前成肌胚胎的分析表明，与核膜相关的是非肌肉型亚型。进一步的免疫荧光和免疫电子显微镜分析表明，这种多肽与核孔复合体相关。这些数据支持了先前提出的假说，即肌球蛋白或肌球蛋白样分子在孔复合体结构中发挥作用。