Strangeways Research Laboratory, Cambridge, England.
J Exp Med. 1963 Jul 1;118(1):1-6. doi: 10.1084/jem.118.1.1.
The effect of excess vitamin A on the oesophageal epithelium of late foetal rats has been studied in organ culture. In explants kept in normal medium the epithelium is, at first, higher and the keratinisation increased as compared with the development of the organ in vivo. At the later stages of growth, the acceleration of keratinisation leads to an extreme thinning of the epithelium. Excess vitamin A completely inhibits keratinisation and induces a transformation of the cells lining the oesophageal lumen into mucin-secreting elements. In vitamin A-treated cultures the epithelium remains high throughout the whole period of cultivation. The amount of secretory matter and the height of the epithelium seem to depend on an adequate supply of oxygen and carbon dioxide to the cells.
过量维生素 A 对晚期胎鼠食管上皮的影响已在器官培养中进行了研究。在正常培养基中培养的外植体中,与体内器官发育相比,上皮最初较高,角化增加。在生长的后期阶段,角化的加速导致上皮极度变薄。过量的维生素 A 完全抑制角化,并诱导食管腔衬里细胞向分泌粘蛋白的细胞转化。在维生素 A 处理的培养物中,上皮在整个培养期间保持较高水平。分泌物质的量和上皮的高度似乎取决于细胞对氧气和二氧化碳的充足供应。