Ben May Laboratory for Cancer Research, The University of Chicago, Chicago.
J Exp Med. 1963 Jul 1;118(1):27-40. doi: 10.1084/jem.118.1.27.
After a single feeding or intravenous injection of 7,12-dimethylbenz[a]anthracene, the testis of rat was severely and selectively damaged, whereas the ovary of sisters was spared from injury. After many weeks, complete recovery of the testis ensued. The destructive effect of 7,12-DMBA on testis was not shared by other powerful carcinogenic hydrocarbons; 3-methylcholanthrene; benzo[a]pyrene; 2-acetaminophenanthrene. The primary sites of destruction inflicted by 7,12-DMBA on testis were spermatogonia and resting spermatocytes, the only cells in testis which synthesize deoxyribose nucleic acid. No other cells in the germinal epithelium are damaged by 7,12-DMBA. The severe atrophy of testis that ensues after some weeks is secondary to destruction of precursors in the seminiferous cell line. Moreover, interstitial cells of testis are not destroyed by the hydrocarbon. Estimation of malic dehydrogenase is a simple and useful quantitative measure of damage and subsequent repair in testis. Following administration of 7,12-DMBA, the level of MDH had an inverse relationship to weight of the testis.
单次经口或静脉注射 7,12-二甲基苯并蒽后，大鼠的睾丸受到严重且选择性的损伤，而其姊妹的卵巢则免受损伤。数周后，睾丸完全恢复。7,12-DMBA 对睾丸的破坏作用并不为其他强效致癌烃；3-甲基胆蒽；苯并[a]芘；2-乙酰氨基菲所共有。7,12-DMBA 对睾丸造成的主要破坏部位是精原细胞和休止期精母细胞，这是睾丸中唯一合成脱氧核糖核酸的细胞。生殖上皮中的其他细胞不受 7,12-DMBA 损害。数周后发生的严重睾丸萎缩是生精细胞系中前体细胞破坏的继发结果。此外，间质细胞不受该烃类破坏。苹果酸脱氢酶的测定是睾丸损伤和随后修复的一种简单而有用的定量测量方法。给予 7,12-DMBA 后，MDH 的水平与睾丸重量呈反比关系。
