Reddy G R, Ueda N, Hada T, Sackeyfio A C, Yamamoto S, Hano Y, Aida M, Nomura T
Department of Biochemistry, Tokushima University School of Medicine, Japan.
Biochem Pharmacol. 1991 Jan 1;41(1):115-8. doi: 10.1016/0006-2952(91)90018-z.
Several naturally occurring prenylflavones were tested for their inhibitory actions on arachidonate 5-lipoxygenase purified from porcine leukocytes. Of the compounds tested, artonin E (5'-hydroxymorusin) exhibited the most potent inhibition on arachidonate 5-lipoxygenase (IC50 = 0.36 microM). Arachidonate 12-lipoxygenase purified from porcine leukocytes and human platelets, 15-lipoxygenase from rabbit reticulocytes and fatty acid cyclooxygenase from bovine vesicular glands were inhibited by the compound only at higher concentrations (IC50 = 2.3, 11, 5.2 and 2.5 microM, respectively).
对几种天然存在的异戊烯基黄酮进行了测试，考察它们对从猪白细胞中纯化得到的花生四烯酸5-脂氧合酶的抑制作用。在所测试的化合物中，阿托尼因E（5'-羟基桑色素）对花生四烯酸5-脂氧合酶表现出最强的抑制作用（IC50 = 0.36微摩尔）。该化合物仅在较高浓度下（IC50分别为2.3、11、5.2和2.5微摩尔）对从猪白细胞和人血小板中纯化得到的花生四烯酸12-脂氧合酶、兔网织红细胞中的15-脂氧合酶以及牛精囊中的脂肪酸环氧化酶有抑制作用。
