Department of Bacteriology and Immunology of Vanderbilt University Medical School, Nashville.
J Exp Med. 1927 Jul 31;46(2):263-77. doi: 10.1084/jem.46.2.263.
The methods previously employed in the study of hemotoxins have been applied in the present investigation to the oxidation and reduction of the bacteriolytic substance of Pneumococcus. It is shown that the bacteriolytic agent, previously inactivated by oxidation, can be "reactivated" by treatment with bacterial reducing agents. Evidence is presented that this "reactivation" represents the reduction of inactive, reversible oxidation products to the original active substance. The bacteriolytic agent is an integral constituent of the pneumococcus cell, which can be separated from the hemotoxin by absorption with red blood cells in the cold.
先前用于研究细胞毒素的方法已应用于本次对肺炎球菌溶菌素的氧化还原研究中。研究表明，先前因氧化而失活的溶菌素可以通过细菌还原剂处理而“复性”。有证据表明，这种“复性”代表了无活性的、可还原的氧化产物还原为原始活性物质。溶菌素是肺炎球菌细胞的一个组成部分，可以通过在冷环境中用红细胞吸附将其与细胞毒素分离。
