Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1931 Jul 31;54(3):295-305. doi: 10.1084/jem.54.3.295.
Experiments are described which confirm the result that the specificity of inhibitory reactions involving substituted aromatic acids is decidedly influenced by the position of the substituent. When antigens with specific groups of very simple constitution are used for the tests, inhibiting effects are obtained also with substances distantly related to those determining the reactivity of the antigens. On the other hand, if antigens are built up from protein and chemical compounds of somewhat higher complexity, the specificity of the inhibition reactions with synthetic crystallized substances is of the same order as that of the usual serum reactions; in other words, it is possible to distinguish such compounds by serological tests as readily as proteins can be differentiated with the aid of precipitating sera.
实验证实，取代芳香酸的抑制反应的特异性确实受到取代基位置的影响。当使用具有非常简单结构的特定基团的抗原进行测试时，与那些决定抗原反应性的物质有一定关系的物质也会产生抑制作用。另一方面，如果抗原是由蛋白质和稍微复杂一些的化学化合物组成的，那么与合成结晶物质的抑制反应的特异性与通常的血清反应相同；换句话说，可以通过血清学测试来区分这些化合物，就像借助沉淀血清来区分蛋白质一样容易。
