Laboratories of the Infantile Paralysis Commission of the Long Island College of Medicine, and the Jewish Hospital, Brooklyn, New York.
J Exp Med. 1936 Jul 31;64(2):173-82. doi: 10.1084/jem.64.2.173.
The positive detection of the virus of poliomyelitis in the nasal secretions of 2 children, 16 and 13 days after the onset of the disease, is described. 7 animals which had been inoculated with other concentrates became ill with symptoms and temperature elevations suggestive of poliomyelitis, from 1 to 3 weeks following inoculation, but without definite paralysis. In 2 of these animals which were sacrificed, the histologic findings were compatible with the diagnosis of poliomyelitis but were not typical. Of the serums of the 5 remaining animals 4 failed to neutralize stock virus, whereas the serum of the fifth neutralized the virus on two different occasions. This serum was obtained from a monkey that had been inoculated with concentrated nasal secretions of a child 43 days after the onset of illness. It is suggested that the present quarantine period of 3 weeks is compatible with the available data. It is further suggested that the methods of procedure described may be useful in similar investigations.
描述了 2 例发病后 16 和 13 天的患儿鼻分泌物中脊灰病毒的阳性检出。7 只接种了其他浓缩物的动物在接种后 1 至 3 周内出现了类似于脊髓灰质炎的症状和体温升高，但没有明确的瘫痪。在 2 只被处死的动物中，组织学发现与脊髓灰质炎的诊断相符，但不典型。在其余 5 只动物的血清中，有 4 只未能中和标准病毒，而第 5 只血清在两次不同的情况下中和了病毒。该血清来自一只猴子，在发病后 43 天接种了患儿的浓缩鼻分泌物。目前的 3 周检疫期与现有数据相符。进一步建议，所描述的程序方法可能对类似的研究有用。
