家禽单纯性鼻炎的研究：VII. 在受精鸡蛋和组织培养物中培养球菌样体。
STUDIES ON AN UNCOMPLICATED CORYZA OF THE DOMESTIC FOWL : VII. CULTIVATION OF THE COCCOBACILLIFORM BODIES IN FERTILE EGGS AND IN TISSUE CULTURES.
机构信息
Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
出版信息
J Exp Med. 1936 Oct 31;64(5):749-58. doi: 10.1084/jem.64.5.749.
The coccobacilliform bodies of fowl coryza were successfully cultivated in the fetal membranes of fertile eggs. Microscopic examination indicated growth in approximately 50 per cent of 94 eggs inoculated on the 3rd to 4th day of incubation. Growth was generally inhibited, however, in eggs inoculated on the 10th day. One strain of the specific bodies was maintained through 11 successive passages in 4 day eggs. A more consistent growth of the coccobacilliform bodies was obtained in tissue cultures. One strain, originally isolated in November, 1935, has been carried through 100 successive subcultures at intervals of 1-3 days. The specific bodies fail to maintain their morphological identity for any length of time in this medium. It is noted that growth of the coccobacilliform bodies in fertile eggs and in tissue cultures is not dependent on the presence of living cells.
鸡传染性鼻炎的杆菌状小体在鸡胚的胎膜中成功培养。显微镜检查表明，在接种后第 3 至第 4 天的 94 个鸡蛋中，约有 50%的鸡蛋中出现了生长。然而，在接种后第 10 天的鸡蛋中，生长通常受到抑制。有一株特定的杆菌状小体在连续 4 天的鸡蛋中通过 11 次传代得到了维持。在组织培养中，获得了更一致的杆菌状小体生长。一株于 1935 年 11 月最初分离的菌株，已经在每隔 1-3 天的时间内连续传代培养了 100 次。在这种培养基中，特定的杆菌状小体无法保持其形态特征达任何一段时间。值得注意的是，杆菌状小体在鸡胚和组织培养中的生长并不依赖于活细胞的存在。
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