Laboratories of the International Health Division, The Rockefeller Foundation, New York.
J Exp Med. 1938 May 31;67(6):941-52. doi: 10.1084/jem.67.6.941.
A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated.
介绍了一种利用 Bauer 和 Pickels 浓度离心机测定蛋白质和其他小颗粒(无论是纯物质还是混合物)相对沉降程度的方法。通过特殊的取样装置,在不同的流体水平下获得的物质进行化学分析或生物测定,用以测量沉降程度。结果在 10%以内具有重现性,并且在所引用的实验条件下,对于迄今为止研究过的蛋白质是有特征性的。