牛痘病毒基本粒子的组成部分：二、疫苗病毒核酸的性质。
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : II. PROPERTIES OF NUCLEIC ACID OBTAINED FROM VACCINE VIRUS.
机构信息
Hospital and Laboratories of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1940 Jul 31;72(2):139-47. doi: 10.1084/jem.72.2.139.
Abstract
It has been possible by means of classical chemical methods to isolate and to characterize to some extent the nucleic acid of elementary bodies of vaccinia. Determination by means of diphenylamine reagent revealed that the major part of the nucleic acid was of the thymus type. This was further substantiated by its stability in the presence of ribonuclease, less than 10 per cent undergoing depolymerization during prolonged incubation at 37 degrees C. By the technique employed, at least 5.6 per cent of the virus was shown to be thymonucleic acid. This amount agreed favorably with the value calculated from the non-lipid organic phosphorus of elementary bodies on the assumption that the phosphorus bound in the organic form was derived principally from nucleic acid.
摘要
通过经典的化学方法，已经有可能分离和在一定程度上鉴定牛痘病毒的基本体的核酸。通过二苯胺试剂的测定表明，核酸的主要部分是胸腺类型。这进一步得到核糖核酸酶存在时的稳定性的证实，在 37°C 长时间孵育时，不到 10%发生解聚。通过采用的技术，至少 5.6%的病毒被证明是胸苷酸。这个数量与根据基本体的非脂类有机磷计算出的值非常吻合，假设结合在有机形式中的磷主要来自核酸。
相似文献
1
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : II. PROPERTIES OF NUCLEIC ACID OBTAINED FROM VACCINE VIRUS.牛痘病毒基本粒子的组成部分：二、疫苗病毒核酸的性质。
J Exp Med. 1940 Jul 31;72(2):139-47. doi: 10.1084/jem.72.2.139.
2
SOME CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA.某些牛痘基本粒子的组成成分。
J Exp Med. 1940 Feb 29;71(3):373-89. doi: 10.1084/jem.71.3.373.
3
IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : III. RESPONSE OF RABBITS TO INACTIVE ELEMENTARY BODIES OF VACCINIA AND TO VIRUS-FREE EXTRACTS OF VACCINE VIRUS.疫苗病毒的免疫学和化学研究：III. 家兔对牛痘的非活性基本体和无病毒疫苗病毒提取物的反应。
J Exp Med. 1936 Jan 1;63(1):69-94. doi: 10.1084/jem.63.1.69.
4
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : V. A FLAVIN ASSOCIATED WITH THE PURIFIED VIRUS.牛痘病毒基本粒子的组成部分：五、一种与提纯病毒有关的黄色素。
J Exp Med. 1941 Jul 31;74(2):133-44. doi: 10.1084/jem.74.2.133.
5
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : III. THE EFFECT OF PURIFIED ENZYMES ON ELEMENTARY BODIES OF VACCINIA.牛痘病毒基本粒子的组成部分：III. 纯化酶对牛痘病毒基本粒子的影响。
J Exp Med. 1940 Nov 30;72(6):685-96. doi: 10.1084/jem.72.6.685.
6
ULTRACENTRIFUGATION STUDIES ON THE ELEMENTARY BODIES OF VACCINE VIRUS : II. THE INFLUENCE OF SUCROSE, GLYCEROL, AND UREA SOLUTIONS ON THE PHYSICAL NATURE OF VACCINE VIRUS.超离心研究疫苗病毒的基本颗粒：二、蔗糖、甘油和尿素溶液对疫苗病毒物理性质的影响。
J Exp Med. 1938 Sep 30;68(4):607-27. doi: 10.1084/jem.68.4.607.
7
IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : I. PREPARATION OF ELEMENTARY BODIES OF VACCINIA.疫苗病毒的免疫学和化学研究：I. 牛痘的基本体的制备。
J Exp Med. 1935 Jun 30;62(1):65-72. doi: 10.1084/jem.62.1.65.
8
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : I. CERTAIN BASIC ANALYSES AND OBSERVATIONS ON LIPID COMPONENTS OF THE VIRUS.牛痘病毒基本粒子的组成成分：I. 对病毒脂类成分的某些基础分析和观察。
J Exp Med. 1940 May 31;71(6):737-50. doi: 10.1084/jem.71.6.737.
9
IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : V. METABOLIC STUDIES OF ELEMENTARY BODIES OF VACCINIA.疫苗病毒的免疫学和化学研究：V. 牛痘基本粒子的代谢研究。
J Exp Med. 1937 Jan 1;65(1):109-20. doi: 10.1084/jem.65.1.109.
10
ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA.疫苗基本体制剂纯度的估计。
J Exp Med. 1939 Sep 30;70(4):379-85. doi: 10.1084/jem.70.4.379.
引用本文的文献
1
Cowpox Viruses: A Zoo Full of Viral Diversity and Lurking Threats.牛痘病毒：病毒多样性的动物园和潜伏的威胁。
Biomolecules. 2023 Feb 8;13(2):325. doi: 10.3390/biom13020325.
2
THE NUCLEIC ACID AND CARBOHYDRATE OF INFLUENZA VIRUS.流感病毒的核酸与碳水化合物。
J Exp Med. 1947 Jan 1;85(1):99-116. doi: 10.1084/jem.85.1.99.
3
CHEMICAL STUDIES ON HOST-VIRUS INTERACTIONS : I. THE EFFECT OF BACTERIOPHAGE ADSORPTION ON THE MULTIPLICATION OF ITS HOST, ESCHERICHIA COLI B WITH AN APPENDIX GIVING SOME DATA ON THE COMPOSITION OF THE BACTERIOPHAGE, T2.宿主-病毒相互作用的化学研究：I. 噬菌体吸附对其宿主大肠杆菌 B 的繁殖的影响，附录中给出了噬菌体 T2 的组成的一些数据。
J Exp Med. 1946 Oct 31;84(5):511-23. doi: 10.1084/jem.84.5.511.
4
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : VI. STUDIES ON THE NATURE OF THE ENZYMES ASSOCIATED WITH THE PURIFIED VIRUS.牛痘病毒基本粒子的组成成分：六、与纯化病毒相关联的酶的性质研究。
J Exp Med. 1942 Aug 1;76(2):163-73. doi: 10.1084/jem.76.2.163.
5
MORPHOLOGICAL STRUCTURE OF THE VIRUS OF VACCINIA.牛痘病毒的形态结构。
J Exp Med. 1942 Jun 1;75(6):651-6. doi: 10.1084/jem.75.6.651.
6
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : V. A FLAVIN ASSOCIATED WITH THE PURIFIED VIRUS.牛痘病毒基本粒子的组成部分：五、一种与提纯病毒有关的黄色素。
J Exp Med. 1941 Jul 31;74(2):133-44. doi: 10.1084/jem.74.2.133.
7
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : IV. DEMONSTRATION OF COPPER IN THE PURIFIED VIRUS.牛痘病毒基本粒子的组成成分：IV. 经提纯的病毒中铜的存在证明。
J Exp Med. 1941 Jun 30;74(1):69-80. doi: 10.1084/jem.74.1.69.
8
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : III. THE EFFECT OF PURIFIED ENZYMES ON ELEMENTARY BODIES OF VACCINIA.牛痘病毒基本粒子的组成部分：III. 纯化酶对牛痘病毒基本粒子的影响。
J Exp Med. 1940 Nov 30;72(6):685-96. doi: 10.1084/jem.72.6.685.
9
Bacteriophage formation without bacterial growth; the effect of iodoacetate, fluoride, gramicidin, and azide on the formation of bacteriophage.无细菌生长情况下的噬菌体形成；碘乙酸盐、氟化物、短杆菌肽和叠氮化物对噬菌体形成的影响。
J Gen Physiol. 1947 Nov 20;31(2):135-9. doi: 10.1085/jgp.31.2.135.
10
Antiviral chemotherapy.抗病毒化疗
Yale J Biol Med. 1956 Sep;29(1):33-49.
本文引用的文献
1
CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : I. CERTAIN BASIC ANALYSES AND OBSERVATIONS ON LIPID COMPONENTS OF THE VIRUS.牛痘病毒基本粒子的组成成分：I. 对病毒脂类成分的某些基础分析和观察。
J Exp Med. 1940 May 31;71(6):737-50. doi: 10.1084/jem.71.6.737.
2
SOME CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA.某些牛痘基本粒子的组成成分。
J Exp Med. 1940 Feb 29;71(3):373-89. doi: 10.1084/jem.71.3.373.
3
ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA.疫苗基本体制剂纯度的估计。
J Exp Med. 1939 Sep 30;70(4):379-85. doi: 10.1084/jem.70.4.379.
4
ISOLATION FROM BEEF PANCREAS OF A CRYSTALLINE PROTEIN POSSESSING RIBONUCLEASE ACTIVITY.从牛胰脏中分离出具有核糖核酸酶活性的结晶蛋白。
Science. 1939 Aug 4;90(2327):112-3. doi: 10.1126/science.90.2327.112.
5
CONCENTRATION AND PURIFICATION OF CHICKEN TUMOR I AGENT.鸡肿瘤I因子的浓缩与纯化
Science. 1938 May 20;87(2264):467-8. doi: 10.1126/science.87.2264.467.
Zotero 插件