Hospital and Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1940 Jul 31;72(2):139-47. doi: 10.1084/jem.72.2.139.
It has been possible by means of classical chemical methods to isolate and to characterize to some extent the nucleic acid of elementary bodies of vaccinia. Determination by means of diphenylamine reagent revealed that the major part of the nucleic acid was of the thymus type. This was further substantiated by its stability in the presence of ribonuclease, less than 10 per cent undergoing depolymerization during prolonged incubation at 37 degrees C. By the technique employed, at least 5.6 per cent of the virus was shown to be thymonucleic acid. This amount agreed favorably with the value calculated from the non-lipid organic phosphorus of elementary bodies on the assumption that the phosphorus bound in the organic form was derived principally from nucleic acid.
通过经典的化学方法,已经有可能分离和在一定程度上鉴定牛痘病毒的基本体的核酸。通过二苯胺试剂的测定表明,核酸的主要部分是胸腺类型。这进一步得到核糖核酸酶存在时的稳定性的证实,在 37°C 长时间孵育时,不到 10%发生解聚。通过采用的技术,至少 5.6%的病毒被证明是胸苷酸。这个数量与根据基本体的非脂类有机磷计算出的值非常吻合,假设结合在有机形式中的磷主要来自核酸。