Departments of Pathology, Radiology, and Pediatrics, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
J Exp Med. 1945 Nov 30;82(6):411-30.
Electrophoretic patterns of human and dog plasma are markedly altered by the extraction of a large part of the plasma lipids. Total electrophoretic areas, relative areas of individual electrophoretic components, and electrophoretic albumin: globulin ratios undergo change. For human plasma, such extractions confirm previous observations that a particularly rich lipid content characterizes beta globulin. Abnormally large beta peaks regularly occur in the presence of elevated plasma lipids. Marked increases in gamma globulin, however, are also found to be due in large part to elevated plasma lipid levels in certain abnormal human plasmas. The greatest relative amount of lipid in dog plasma, in contrast to human plasma, is associated not with the beta globulin, but with components usually designated as alpha globulins. Not only the areas, but the configuration and the number of alpha globulin peaks in dog plasma are altered by the extraction of plasma lipids. The results demonstrate that increased alpha globulin areas which occur in the plasma of hypoproteinemic dogs are due in large part to elevated plasma lipid levels.
人血浆和犬血浆的电泳图谱因大部分血浆脂质被提取而明显改变。总电泳面积、各电泳成分的相对面积以及电泳白蛋白与球蛋白比值均发生变化。对于人血浆，这种提取方法证实了先前的观察结果，即β球蛋白的脂质含量特别丰富。在血浆脂质升高的情况下，通常会出现异常大的β峰。然而，还发现γ球蛋白的显著增加在很大程度上也是由于某些异常人血浆中血浆脂质水平升高所致。与人类血浆相比，犬血浆中相对脂质含量最大的不是β球蛋白，而是通常被指定为α球蛋白的成分。不仅是面积，而且犬血浆中α球蛋白峰的形态和数量也因提取血浆脂质而发生改变。结果表明，低蛋白血症犬血浆中出现的α球蛋白面积增加在很大程度上是由于血浆脂质水平升高所致。
