Moran A P, Rietschel E T, Kosunen T U, Zähringer U
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.
J Bacteriol. 1991 Jan;173(2):618-26. doi: 10.1128/jb.173.2.618-626.1991.
Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (H. Brade and C. Galanos, Eur. J. Biochem. 122:233-237, 1982) gave better yields of LPS than did extraction by the conventional hot phenol-water technique. Constituents common to all LPS were D-glucose, D-galactose, L-glycero-D-manno-heptose, 3-deoxy-D-manno-2-octulosonic acid, D-glucuronic acid, D-galactosamine, and phosphorylethanolamine. Phosphate was present in a relatively high amount. In addition, the LPS of three strains contained N-acetylneuraminic acid, whereas the LPS of the strain lacking this component contained 3-amino-3,6-dideoxy-D-glucose. The lipid A component contained phosphate with D-glucosamine and 2,3-diamino-2,3-dideoxy-D-glucose as the major amino sugars. Ethanolamine-phosphate was present also. The major fatty acids were ester- and amide-bound 3-hydroxytetradecanoic and ester-bound hexadecanoic acids, with a minor amount of ester-bound tetradecanoic acid. This is the first report of N-acetylneuraminic acid in the oligosaccharide moiety and diaminoglucose in the lipid A of C. jejuni LPS.
对人类肠道病原体空肠弯曲菌的四种非荚膜菌株的脂多糖（LPS）进行了化学特性分析。将改良的苯酚-氯仿-石油醚法（H. 布拉德和C. 加拉诺斯，《欧洲生物化学杂志》122:233 - 237，1982年）应用于其中两种菌株时，与传统的热酚-水技术相比，LPS的产量更高。所有LPS共有的成分有D-葡萄糖、D-半乳糖、L-甘油-D-甘露庚糖、3-脱氧-D-甘露-2-辛酮糖酸、D-葡萄糖醛酸、D-半乳糖胺和磷酸乙醇胺。磷酸盐含量相对较高。此外，三种菌株的LPS含有N-乙酰神经氨酸，而缺乏该成分的菌株的LPS含有3-氨基-3,6-二脱氧-D-葡萄糖。脂质A成分含有磷酸盐，主要氨基糖为D-葡萄糖胺和2,3-二氨基-2,3-二脱氧-D-葡萄糖。也存在乙醇胺-磷酸盐。主要脂肪酸为酯键和酰胺键连接的3-羟基十四烷酸以及酯键连接的十六烷酸，还有少量酯键连接的十四烷酸。这是关于空肠弯曲菌LPS的寡糖部分中存在N-乙酰神经氨酸以及脂质A中存在二氨基葡萄糖的首次报道。
