Department of Bacteriology, New York University College of Medicine, New York.
J Exp Med. 1947 May 31;85(6):701-13. doi: 10.1084/jem.85.6.701.
Maximum production of the alpha toxin by Cl. welchii is dependent on the inclusion in the medium of several substances in addition to those required for growth. These factors include: 1. Some substance present in enzymatic digests of certain proteins such as casein and gelatine. 2. Glycerylphosphorylcholine and other substances that are present in extracts of pancreas. 3. The use of starch or dextrin as a carbohydrate source in the absence of other fermentable carbohydrates. The omission of any one of these factors from the medium results in a very low yield of alpha toxin.
产α毒素梭菌的最大产量除了生长所需的物质外,还依赖于培养基中包含的几种物质。这些因素包括:1. 某些蛋白质(如酪蛋白和明胶)酶解产物中的某些物质。2. 甘油磷酸胆碱和其他存在于胰腺提取物中的物质。3. 在没有其他可发酵碳水化合物的情况下,将淀粉或糊精用作碳水化合物来源。培养基中缺少这些因素中的任何一种都会导致α毒素的产量非常低。