Departments of Biology and Pathology, St. Louis University, St. Louis.
J Exp Med. 1950 Feb 28;91(3):335-9. doi: 10.1084/jem.91.3.335.
Allantoic fluid from embryonated eggs infected with influenza A virus contains a toxic agent which can be demonstrated and quantitatively measured by its rapid effect on oxygen consumption when it is introduced in new series of fertile eggs. The effects were measured 90 minutes after the injection of the infected fluid, and were seen following both intra-allantoic injection and injection into the yolk sac. This toxin, in concentrations resulting from the injection of 0.5 cc. or less of the infected fluid, has no effect on oxygen consumption. The injection of 0.75 to 2.0 cc. of the fluid strikingly increases the oxygen consumption of the fertile eggs, while the injection of 3.0 cc. markedly depresses respiration. A similar reversal and eventual loss of the effect of the toxin on respiration were noted when the concentration of toxin was progressively diminished by heat inactivation. The toxic agent is slowly inactivated by heating at 56 degrees C., but is effective long after infectivity and hemagglutinating ability have been destroyed. In this respect the agent differs from rickettsial and lymphogranuloma venereum virus toxins. The method described may be of value in studying the physiological effects of other toxic agents.
鸡胚羊水感染流感病毒后含有一种有毒物质,这种物质可以通过其对新系列受精卵耗氧量的快速影响来证明和定量测量。在感染液注射 90 分钟后测量效果,在羊膜腔内注射和蛋黄囊内注射后都可以观察到。这种毒素在注射 0.5 毫升或更少的感染液时,其浓度不会影响耗氧量。注射 0.75 至 2.0 毫升的溶液会显著增加受精卵的耗氧量,而注射 3.0 毫升则明显抑制呼吸。当毒素浓度通过热失活逐渐降低时,观察到毒素对呼吸的作用发生类似的逆转和最终丧失。加热至 56°C 时,有毒物质的失活速度较慢,但在感染力和血凝能力丧失后仍具有活性。在这方面,该试剂与立克次体和淋巴肉芽肿病毒毒素不同。所描述的方法可能对研究其他有毒物质的生理效应有价值。