Karaivanov L
Vet Med Nauki. 1977;14(1):3-7.
Studied was the adsorption of 8 strains of Pasteurella multocida bacteriophages presenting three groups of phages isolated in Bulgaria. With the bacteriophages of groups I and II the adsorption in broth in the first 10 minutes amounted to 50-65%, and at the 80 the minute reached its maximum value--98-99%. With the bacteriophages of group III adsorption started with a lower percent and by the 140th minute reached 96-97%. It was by 60 min slower as compared with the adsorption with group I and II phages. In a veronal-medinal buffer the adsorption of all investigated phages of the three groups set in with a prolonged time of 60 min as compared with the time elapsed using broth. At the cross testing of adsorption the phages of group I were adsorbed by the hosts of the other two groups to a maximum level, however, the phages of groups II and III were adsorbed to a negligible extent on the other hosts (except for the adsorption on their homologous one). The difference established in the adsorption for a 60 minutes time so far as the phages of group III were concerned was sufficiently persuasive to consider this group as an individual one by this feature too. Adsorption appeared as a subsidiary taxonomic characteristic in the classification of Pasteurella multocida bacteriophages.
对8株多杀性巴氏杆菌噬菌体的吸附情况进行了研究,这8株噬菌体代表了在保加利亚分离出的三组噬菌体。对于I组和II组的噬菌体,在肉汤中10分钟内的吸附率为50%-65%,到80分钟时达到最大值——98%-99%。对于III组的噬菌体,吸附开始时的百分比较低,到140分钟时达到96%-97%。与I组和II组噬菌体的吸附相比,其吸附速度慢60分钟。在韦罗那-梅迪纳缓冲液中,与使用肉汤时相比,三组所有研究噬菌体的吸附时间延长至60分钟。在交叉吸附试验中,I组噬菌体被其他两组的宿主最大程度地吸附,然而,II组和III组噬菌体在其他宿主上的吸附程度可忽略不计(除了在其同源宿主上的吸附)。就III组噬菌体而言,60分钟吸附过程中所确定的差异足以令人信服地认为该组在这一特征上也属于一个独立的组。吸附在多杀性巴氏杆菌噬菌体分类中表现为一种辅助分类特征。