Laboratories of Physiology and Biochemistry, Massachusetts Institute of Technology Cambridge.
J Gen Physiol. 1941 May 20;24(5):655-67. doi: 10.1085/jgp.24.5.655.
1.Fusarium tricothecoides was selected for a study of the respiratory and fermentative activities of Fusaria. "Resting cell" suspensions were investigated by the Barcroft manometric technique. 2. The results of the investigation indicate clearly that the mechanism of endogenous metabolism (respiration) is distinct from the exogenous mechanism (fermentation). Anaerobically no significant CO(2) production is apparent without added substrate. In the presence of glucose the anaerobic CO(2) evolution is practically equal to the added CO(2) evolved aerobically in the presence of added glucose. Low concentrations of iodoacetate or fluoride selectively poison the exogenous mechanism but do not affect the endogenous mechanism. Alcohol is not produced in the course of endogenous metabolism, but is produced in the presence of added glucose. 3. A study of the metabolism of the organism throughout its entire growth phase from 1 to 7 days has been made. 4. The ability of suspensions of Fusarium sp. H., obtained by growth on a variety of common substrates, to attack a large number of carbon sources with the production of exogenous CO(2) was determined. It is found that organisms grown on glucose will attack only glucose, mannose, and fructose, but none of the common intermediary metabolites except pyruvic acid. Organisms grown on galactose attack galactose, as well as the other hexoses, indicating an adaptive mechanism. 5. An identical mechanism for the dissimilation of glucose, mannose, and galactose is indicated since no additive effects with these substrates were observed. Growths on non-hexose carbon sources attack glucose slightly under the experimental conditions with the evolution of CO(2), but do not attack any other substrate. This would indicate a residual glucose-dissimilating mechanism in all growths investigated. 6. Striking similarities between the general metabolism of resting suspensions of Fusarium sp. H. and resting suspensions of yeast cells are apparent.
选择镰孢菌属真菌（ Fusarium tricothecoides ）来研究镰孢菌的呼吸和发酵活动。通过 Barcroft 测压技术研究“休止细胞”悬浮液。
研究结果清楚地表明，内源性代谢（呼吸）机制与外源性机制（发酵）不同。在没有添加底物的情况下，无氧条件下没有明显的 CO 2 产生。在添加葡萄糖的情况下，无氧条件下 CO 2 的释放几乎与有氧条件下添加葡萄糖时释放的 CO 2 相等。低浓度的碘乙酰胺或氟化物选择性地抑制外源性机制，但不影响内源性机制。在内源性代谢过程中不产生酒精，但在添加葡萄糖的情况下会产生酒精。
对该生物体在整个生长阶段（1 至 7 天）的代谢进行了研究。
测定了从各种常见基质上生长的 Fusarium sp. H. 的悬浮液在产生外源性 CO 2 的情况下攻击大量碳源的能力。结果发现，在葡萄糖上生长的生物体仅攻击葡萄糖、甘露糖和果糖，而除丙酮酸外，不攻击任何其他常见中间代谢物。在半乳糖上生长的生物体攻击半乳糖以及其他己糖，表明存在适应性机制。
由于没有观察到这些底物的累加效应，因此表明葡萄糖、甘露糖和半乳糖的异化作用具有相同的机制。在实验条件下，以非己糖碳源生长的生物体略微攻击葡萄糖以释放 CO 2，但不攻击任何其他底物。这表明在所研究的所有生长中都存在残留的葡萄糖异化机制。
静止的 Fusarium sp. H. 悬浮液和静止的酵母细胞的一般代谢之间存在明显的相似之处。
