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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.

J Gen Physiol. 1941 Nov 20;25(2):185-95. doi: 10.1085/jgp.25.2.185.

Pepsin solutions were iodinated at pH 5.0-6.0 until 10-20 per cent of the activity was lost and 1/20 (0.7 per cent) of the saturating amount of iodine had been introduced into the protein molecule. After alkaline hydrolysis 65 per cent of the original iodine was accounted for as mono-iodotyrosine although only 42 per cent was isolated as a crystalline product. No evidence was obtained to support the possibility that any group other than tyrosine in pepsin was iodinated. 2. Some of the properties of the crystalline l-mono-iodotyrosine were determined and compared to those of di-iodotyrosine. 3. One iodinated pepsin preparation was crystallized. The crystal form was the same as that of the original pepsin. A solubility curve of the crystals demonstrated that it was very different from pepsin and had nearly constant solubility.

在 pH 值 5.0-6.0 下，胃蛋白酶溶液被碘化，直到 10-20%的活性丧失，并且 1/20（0.7%）的饱和碘量被引入蛋白质分子中。碱性水解后，65%的原始碘被认为是单碘酪氨酸，尽管只有 42%被分离为结晶产物。没有证据表明胃蛋白酶中的酪氨酸以外的任何基团被碘化。
确定了结晶 l-单碘酪氨酸的一些性质，并与二碘酪氨酸的性质进行了比较。
一种碘化胃蛋白酶制剂被结晶。晶体形式与原始胃蛋白酶相同。晶体的溶解度曲线表明，它与胃蛋白酶非常不同，几乎具有恒定的溶解度。

Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.

J Gen Physiol. 1941 Nov 20;25(2):185-95. doi: 10.1085/jgp.25.2.185.

Pepsin solutions were iodinated at pH 5.0-6.0 until 10-20 per cent of the activity was lost and 1/20 (0.7 per cent) of the saturating amount of iodine had been introduced into the protein molecule. After alkaline hydrolysis 65 per cent of the original iodine was accounted for as mono-iodotyrosine although only 42 per cent was isolated as a crystalline product. No evidence was obtained to support the possibility that any group other than tyrosine in pepsin was iodinated. 2. Some of the properties of the crystalline l-mono-iodotyrosine were determined and compared to those of di-iodotyrosine. 3. One iodinated pepsin preparation was crystallized. The crystal form was the same as that of the original pepsin. A solubility curve of the crystals demonstrated that it was very different from pepsin and had nearly constant solubility.

在 pH 值 5.0-6.0 下，胃蛋白酶溶液被碘化，直到 10-20%的活性丧失，并且 1/20（0.7%）的饱和碘量被引入蛋白质分子中。碱性水解后，65%的原始碘被认为是单碘酪氨酸，尽管只有 42%被分离为结晶产物。没有证据表明胃蛋白酶中的酪氨酸以外的任何基团被碘化。
确定了结晶 l-单碘酪氨酸的一些性质，并与二碘酪氨酸的性质进行了比较。
一种碘化胃蛋白酶制剂被结晶。晶体形式与原始胃蛋白酶相同。晶体的溶解度曲线表明，它与胃蛋白酶非常不同，几乎具有恒定的溶解度。