Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada.
J Gen Physiol. 1968 Jul 1;52(1):173-86.
The molecular arrangement within a lamellar structure composed of human erythrocyte lipids is determined. The 45 A thick lipid layer, in water, is filled in the interior with a liquid-like configuration of the hydrocarbon chains of phospholipid molecules and is covered on both sides by their hydrophilic polar groups. Cholesterol is located so that part of its steroid nucleus is between the polar groups of the phospholipid molecules while the rest of the molecule extends into the inner hydrocarbon layer. This lipid leaflet would be expected to have the mechanical properties of a purely liquid surface, as other authors have shown for the "black" lipid membranes. Data are presented which demonstrate that the intact erythrocyte membrane is a tough viscoelastic substance with a Young's modulus of 10(6)-10(8) dynes/cm(2) and a viscosity of 10(7)-10(10) poises. The parameters and the kinetics of membrane breakdown are incompatible with the model system of pure lipid. Caution must be exercised in applying various data on the model systems to intact membranes.
确定了由人红细胞脂质组成的层状结构内的分子排列。在水中，45 A 厚的脂质层内部充满了磷脂分子的烃链的液态构型，并且在两侧被其亲水性极性基团覆盖。胆固醇位于这样的位置，即其甾体核的一部分位于磷脂分子的极性基团之间，而分子的其余部分延伸到内部烃层中。如其他作者为“黑色”脂质膜所示，这种脂质小叶预计将具有纯液态表面的机械特性。本文提供的数据表明，完整的红细胞膜是一种坚韧的粘弹性物质，杨氏模量为 10(6)-10(8)达因/平方厘米，粘度为 10(7)-10(10)泊。膜破裂的参数和动力学与纯脂质模型系统不兼容。在将各种模型系统的数据应用于完整膜时必须小心谨慎。
