[南京某钾矿尾矿库优势植物可培养细菌的遗传多样性]

[Genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of Nanjing].

作者信息

Wang Jing, Sheng Xiafang, Cao Jianfang, Zhang Shukui, Zhang Yin, He Linyan

机构信息

College of Life Science, Nanjing Agriculture University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China.

出版信息

Wei Sheng Wu Xue Bao. 2009 Jul;49(7):867-73.

PMID:19873749

Abstract

OBJECTIVE

Plant rhizosphere bacteria play an important role in biogeochemical cycles. Microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.

METHODS

Cultivable bacteria were isolated by plating and screening from plant root, rhizosphere and bulk soils of predominant plants in a mine tailing of Nanjing, Jiangsu Province. Phylogenetic analyse based on 16S rDNA sequence comparisons and amplified rDNA restriction analysis of isolates were investigated.

RESULTS

In total, 60 pure cultures were isolated; they could be grouped into 18 different operational taxonomic units (OTU) at the similarity level of 60%. Nineteen bacterial strains belonged to eleven genera (Rhizobium, Pseudomonas, Pantoea, Arthrobacter, Microbacterium, Bacillus, Paenibacillus, Acinetobacter, Sphingomonas, Kocuria , Mitsuaria) of three major phylogenetic groups (Proteobacteria, Actinobacteria, Firmicutes). Rhizobium, Pseudomonas and Pantoea were the dominant groups.

CONCLUSION

Different cultivable bacteria inhabited in roots and soils of dominant plants in the mine tailing. They might play a certain role in the soil-plant-mineral environment.

摘要

目的

植物根际细菌在生物地球化学循环中发挥着重要作用。对江苏南京某尾矿土壤-植物-矿物系统中可培养细菌的微生物多样性进行了评估。

方法

通过平板培养和筛选，从江苏省南京市某尾矿优势植物的根、根际和块状土壤中分离可培养细菌。基于16S rDNA序列比较对分离菌株进行系统发育分析，并对其进行扩增rDNA限制性分析。

结果

共分离出60株纯培养物；在60%的相似性水平上，它们可分为18个不同的操作分类单元（OTU）。19株细菌菌株属于三大系统发育类群（变形菌门、放线菌门、厚壁菌门）的11个属（根瘤菌属、假单胞菌属、泛菌属、节杆菌属、微杆菌属、芽孢杆菌属､类芽孢杆菌属、不动杆菌属、鞘氨醇单胞菌属、考克氏菌属、水栖菌属）。根瘤菌属、假单胞菌属和泛菌属为优势类群。