Sun Leni, He Linyan, Zhang Yanfeng, Zhang Wenhui, Wang Qi, Sheng Xiafang
College of Life Science, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China.
Wei Sheng Wu Xue Bao. 2009 Oct;49(10):1360-6.
Isolation and characterization of rhizosphere copper-resistant bacteria from a copper accumulator plant Elsholtzia splendens were investigated.
Cultivable Cu-resistant bacteria were isolated by plating and screening from rhizosphere soils of Elsholtzia splendens growing on a copper mine tailing. Bacteria were characterized regarding characteristics that may be relevant for a beneficial plant-microbe interaction--Cu tolerance, phosphate-solubilizing, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophore and indoleacetic acid production, and further classified by restriction analysis of 16S rDNA (ARDRA). Strains that produced ACC deaminase were identified by 16S rDNA sequence analysis.
Twenty-seven Cu-resistant strains were isolated from rhizosphere soil of Elsholtzia splendens and classified by ARDRA in 7 different taxonomic groups at the similarity level of 60% . All strains produced IAA or their derivatives, 44.4% of the strains produced a very high level of siderophores, and five strains were able to grow on ACC as the sole nitrogen source. Strains 2EBS12, 2EBS13, 2EBS15 and 3EBS11 were identified as Acinetobacter, strain 2EBS14 was essentially consistent Alcaligenes.
Cu-resistant rhizobacteria isolated from Elsholtzia splendens have abundant characteristics relative to promoting plant growth and genetic diversity, rhizobacteria Acinetobacter sp. and Alcaligenes sp. contained ACC deaminase activity.
研究从铜积累植物海州香薷根际分离筛选耐铜细菌并对其进行鉴定。
通过平板划线法从生长在铜尾矿上的海州香薷根际土壤中分离可培养的耐铜细菌，并对其与植物-微生物有益互作相关的特性进行鉴定，包括耐铜性、解磷能力、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性、铁载体和吲哚乙酸的产生情况，进一步通过16S rDNA限制性分析（ARDRA）进行分类。对产生ACC脱氨酶的菌株进行16S rDNA序列分析鉴定。
从海州香薷根际土壤中分离出27株耐铜菌株，通过ARDRA在相似性水平为60%时分为7个不同的分类群。所有菌株都产生吲哚乙酸或其衍生物，44.4%的菌株产生高水平的铁载体，5株菌株能够以ACC作为唯一氮源生长。菌株2EBS12、2EBS13、2EBS15和3EBS11被鉴定为不动杆菌属，菌株2EBS14基本与产碱杆菌一致。
从海州香薷中分离的耐铜根际细菌在促进植物生长和遗传多样性方面具有丰富的特性，不动杆菌属和产碱杆菌属的根际细菌含有ACC脱氨酶活性。
