Cui Zhisong, Shao Zongze
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Wei Sheng Wu Xue Bao. 2009 Jul;49(7):902-9.
In order to identify the predominant strains of polycyclic aromatic hydrocarbon (PAH)-degrading consortia harboring in sea water and surface sediment collected from deep sea of the Middle Atlantic Ridge.
We employed enrichment method and spread-plate method to isolate cultivable bacteria and PAHs degraders from deep sea samples. Phylogenetic analysis was conducted by 16S rRNA gene sequencing of the bacteria. Then we analyzed the dominant bacteria in the PAHs-degrading consortia by denaturing gradient gel electrophoresis (DGGE) combined with DNA sequencing.
Altogether 16 cultivable bacteria were obtained, including one PAHs degrader Novosphingobium sp. 4D. Phylogenetic analysis showed that strains closely related to Alcanivorax dieselolei NO1A (5/16) and Tistrella mobilis TISTR 1108T (5/16) constituted two biggest groups among the cultivable bacteria. DGGE analysis showed that strain 4L (also 4M and 4N, Alcanivorax dieselolei NO1A, 99.21%), 4D (Novosphingobium pentaromativorans US6-1(T), 97.07%) and 4B (also 4E, 4H and 4K, Tistrella mobilis TISTR 1108T, > 99%) dominated the consortium MC2D. While in consortium MC3CO, the predominant strains were strain 5C (also 5H, Alcanivorax dieselolei NO1A, > 99%), uncultivable strain represented by band 5-8 (Novosphingobium aromaticivorans DSM 12444T, 99.41%), 5J (Tistrella mobilis TISTR 1108T, 99.52%) and 5F (also 5G, Thalassospira lucentensis DSM 14000T, < 97%).
We found that strains of genus Alcanivorax, Novosphingobium, Tistrella and Thalassospira were predominant bacteria of PAHs-degrading consortia in sea water and surface sediment of Middle Atlantic Ridge deep sea, with Novosphingobium spp. as their main PAHs degraders.
鉴定来自中大西洋海岭深海海水和表层沉积物中多环芳烃（PAH）降解菌群的优势菌株。
我们采用富集培养法和平板涂布法从深海样本中分离可培养细菌和PAH降解菌。通过对细菌的16S rRNA基因测序进行系统发育分析。然后，我们结合变性梯度凝胶电泳（DGGE）和DNA测序分析PAH降解菌群中的优势细菌。
共获得16株可培养细菌，其中包括1株PAH降解菌新鞘氨醇菌属（Novosphingobium sp.）4D。系统发育分析表明，与嗜油栖热菌属（Alcanivorax dieselolei）NO1A（5/16）和运动蒂氏菌（Tistrella mobilis）TISTR 1108T（5/16）密切相关的菌株在可培养细菌中构成两个最大的类群。DGGE分析表明，菌株4L（也包括4M和4N，嗜油栖热菌属NO1A，相似度99.21%）、4D（新鞘氨醇菌属（Novosphingobium pentaromativorans）US6-1(T)，相似度97.07%）和4B（也包括4E、4H和4K，运动蒂氏菌TISTR 1108T，相似度>99%）在菌群MC2D中占主导地位。而在菌群MC3CO中，优势菌株为菌株5C（也包括5H，嗜油栖热菌属NO1A，相似度>99%）、以条带5-8代表的不可培养菌株（芳香新鞘氨醇菌（Novosphingobium aromaticivorans）DSM 12444T，相似度99.41%）、5J（运动蒂氏菌TISTR 1108T，相似度99.52%）和5F（也包括5G，明亮海螺旋菌（Thalassospira lucentensis）DSM 14000T，相似度<97%）。
我们发现嗜油栖热菌属、新鞘氨醇菌属、蒂氏菌属和海螺旋菌属的菌株是中大西洋海岭深海海水和表层沉积物中PAH降解菌群的优势细菌，其中新鞘氨醇菌属是主要的PAH降解菌。
