Department of Oncology, Nanjing Drum Tower Hospital, Nanjing University of Traditional Chinese Medicine, Zhongshan Road 321, Nanjing 210008, China.
Evid Based Complement Alternat Med. 2011;2011:834231. doi: 10.1093/ecam/nep175. Epub 2011 Jun 5.
The herb medicine formula "Chong Lou Fu Fang" (CLFF) has efficacy in inhibiting the proliferation of human gastric cancer in vitro and in vivo. To explore the potentially useful combination of CLFF with chemotherapeutic agents commonly used in gastric cancer therapy, we assess the interaction between CLFF and these chemotherapeutic agents in both SGC-7901 cell lines and BGC-823 cell lines using a median effect analysis and apoptosis analysis, and we also investigate the influence of CLFF on chemotherapeutic agent-associated gene expression. The synergistic analysis indicated that CLFF had a synergistic effect on the cytotoxicity of 5-fluorouracil (5-FU) in a relative broad dose inhibition range (20-95% fraction affected in SGC-7901cell lines and 5-65% fraction affected in BGC-823 cell lines), while the synergistic interaction between CLFF and oxaliplatin or docetaxel only existed in a low dose inhibition range (≤50% fraction affected in both cell lines). Combination of CLFF and chemotherapeutic agents could also induce apoptosis in a synergistic manner. After 24 h, CLFF alone or CLFF combination with chemotherapeutic agents could significantly suppress the levels of expression of chemotherapeutic agent resistance related genes in gastric cancer cells. Our findings indicate that there are useful synergistic interactions between CLFF and chemotherapeutic agents in gastric cancer cells, and the possible mechanisms might be partially due to the down-regulation of chemotherapeutic agent resistance related genes and the synergistic apoptotic effect.
草药方剂“虫楼复方”（CLFF）在体外和体内均能有效抑制人胃癌的增殖。为了探索 CLFF 与胃癌治疗中常用的化疗药物的潜在联合应用，我们采用中效分析和凋亡分析评估 CLFF 与这些化疗药物在 SGC-7901 细胞系和 BGC-823 细胞系中的相互作用，并研究 CLFF 对化疗药物相关基因表达的影响。协同分析表明，CLFF 在相对较宽的剂量抑制范围内（20-95%受影响分数，在 SGC-7901 细胞系中；5-65%受影响分数，在 BGC-823 细胞系中）对 5-氟尿嘧啶（5-FU）的细胞毒性具有协同作用，而 CLFF 与奥沙利铂或多西他赛之间的协同相互作用仅存在于低剂量抑制范围内（≤50%受影响分数，在两种细胞系中）。CLFF 与化疗药物联合使用也可以协同诱导细胞凋亡。24 h 后，CLFF 单独或与化疗药物联合使用均可显著抑制胃癌细胞中化疗药物耐药相关基因的表达水平。我们的研究结果表明，CLFF 与化疗药物在胃癌细胞中存在有用的协同相互作用，其可能的机制部分归因于下调化疗药物耐药相关基因和协同凋亡作用。
