Organisciak D T, Klingman J D, Tanski W A
Prep Biochem. 1977;7(3-4):217-23. doi: 10.1080/00327487708061639.
An apparatus for the preparation of uniform thin layer chromatography gel layers is described. The apparatus employs rubber cushion runners to compensate for differences in plate thickness and a gel applicator which functions independently of plate edge variability. Consistently uniform layers are prepared by careful establishment of the plate-to-applicator distance. Silica gel layers averaged 87% of the applied thickness with a variability of +/-2% within a single run and +/-8% between independent runs.
描述了一种用于制备均匀薄层色谱凝胶层的装置。该装置采用橡胶缓冲滚轮来补偿板厚度的差异,以及一个独立于板边缘变化起作用的凝胶涂布器。通过仔细确定板与涂布器之间的距离来制备始终均匀的层。硅胶层平均为施加厚度的87%,单次运行内的变化率为±2%,独立运行之间的变化率为±8%。