突尼斯献血者（门诊患者）、有症状患者及对照人群中幽门螺杆菌的血清阳性率。

Seroprevalence of Helicobacter pylori among Tunisian blood donors (outpatients), symptomatic patients and control subjects.

作者信息

Mansour K B, Keita A, Zribi M, Masmoudi A, Zarrouk S, Labbene M, Kallel L, Karoui S, Fekih M, Matri S, Boubaker J, Cheikh I, Chouaib S, Filali A, Mami N B, Najjar T, Fendri C

机构信息

Laboratoire de microbiologie, UR04SP08, CHU Rabta, 1007 El Jebbari, Tunis, Tunisia. khansa

出版信息

Gastroenterol Clin Biol. 2010 Jan;34(1):75-82. doi: 10.1016/j.gcb.2009.06.015. Epub 2009 Oct 29.

DOI:10.1016/j.gcb.2009.06.015

Abstract

OBJECTIVES

Helicobacter pylori is a worldwide infection, although little data are available in the Tunisian population. The aims of our study were to detect the prevalence of H. pylori in a blood-donor population (n=250) and in another population of hospital-consulting patients comprising 87 symptomatic patients and 59 controls, and to determine the factors that influence the prevalence.

MATERIALS AND METHODS

Study subjects answered a standardized questionnaire, and IgG anti-H. pylori and anti-cag were detected by ELISA. In the second population, culture and cagA polymerase chain reaction were performed.

RESULTS

The seroprevalence of H. pylori in blood donors was 64%, and 11% had anti-cag. All patients positive for anti-cag were also positive for anti-H. pylori antibodies. The seroprevalence of H. pylori was 99.3% in the hospital-consulting patients, of whom 55.5% were positive for anti-cag. The difference between the anti-cag and symptomatic patients (66.7%) and controls (39%) was significant. Symptomatic patients had a higher rate of anti-cag (66.7%) compared with the controls (39%) and blood donors (11%).

CONCLUSION

H. pylori seroprevalence in blood donors is low (64%) compared with symptomatic patients (99.3%), and anti-cag was statistically associated with symptomatic patients and pathology. Also, some environmental factors were correlated with H. pylori seroprevalence.

摘要

目的

幽门螺杆菌感染在全球范围内普遍存在，不过关于突尼斯人群的相关数据较少。我们研究的目的是检测献血者人群（n = 250）以及另一组由87名有症状患者和59名对照组成的医院门诊患者人群中幽门螺杆菌的感染率，并确定影响感染率的因素。

材料与方法

研究对象回答一份标准化问卷，采用酶联免疫吸附测定法检测抗幽门螺杆菌IgG和抗细胞毒素相关基因A（cagA）抗体。对第二组人群进行培养和cagA聚合酶链反应检测。

结果

献血者中幽门螺杆菌血清阳性率为64%，11%的人有抗cagA抗体。所有抗cagA阳性患者的抗幽门螺杆菌抗体也呈阳性。医院门诊患者中幽门螺杆菌血清阳性率为99.3%，其中55.5%的人抗cagA呈阳性。抗cagA抗体在有症状患者（66.7%）和对照（39%）之间的差异具有统计学意义。与对照（39%）和献血者（11%）相比，有症状患者的抗cagA抗体阳性率更高（66.7%）。

结论

与有症状患者（99.3%）相比，献血者中幽门螺杆菌血清阳性率较低（64%），抗cagA抗体与有症状患者及病理情况存在统计学关联。此外，一些环境因素与幽门螺杆菌血清阳性率相关。