Australian Pulp and Paper Institute, Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
Colloids Surf B Biointerfaces. 2010 Feb 1;75(2):441-7. doi: 10.1016/j.colsurfb.2009.09.032. Epub 2009 Sep 30.
The feasibility of thermal ink jet printing as a robust process for biosurface engineering was demonstrated. The strategy investigated was to reconstruct a commercial printer and take advantage of its colour management interface. High printing resolution was achieved by formulating bio-inks of viscosity and surface tension similar to those of commercial inks. Protein and enzyme denaturation during thermal ink jet printing was shown to be insignificant. This is because the time spent by the biomolecules in the heating zone of the printer is negligible; in addition, the air and substrate of high heat capacity absorb any residual heat from the droplet. Gradients of trophic/tropic factors can serve as driving force for cell growth or migration for tissue regeneration. Concentration gradients of proteins were printed on scaffolds to show the capability of ink jet printing. The printed proteins did not desorb upon prolonged immersion in aqueous solutions, thus allowing printed scaffold to be used under in vitro and in vivo conditions. Our group portrait was ink jet printed with a protein on paper, illustrating that complex biopatterns can be printed on large area. Finally, patterns of enzymes were ink jet printed within the detection and reaction zones of a paper diagnostic.
热喷墨打印作为一种稳健的生物表面工程处理方法的可行性已得到证实。研究中采用的策略是改造商用打印机并利用其色彩管理接口。通过将生物墨水的粘度和表面张力调整至与商用墨水相似，实现了高打印分辨率。实验表明，在热喷墨打印过程中蛋白质和酶不会发生变性。这是因为生物分子在打印机加热区停留的时间非常短；此外，具有高热容量的空气和基底会吸收来自液滴的任何余热。营养/趋化因子的浓度梯度可以作为细胞生长或组织再生迁移的驱动力。我们在支架上打印了蛋白质浓度梯度，以展示喷墨打印的能力。打印的蛋白质在长时间浸泡在水溶液中也不会解吸，因此允许打印支架在体外和体内条件下使用。我们组的肖像被喷墨打印在纸上的蛋白质上，说明复杂的生物图案可以打印在大面积上。最后，我们在纸张诊断的检测和反应区喷墨打印了酶的图案。
