Brady K, Duff P, Harlass F E, Reid S
Division of Maternal-Fetal Medicine, Madigan Army Medical Center, Tacoma, Washington 98431-5418.
Am J Perinatol. 1991 Jan;8(1):68-70. doi: 10.1055/s-2007-999345.
Chromosome abnormalities may be present in approximately 10% of cases of fetal death. Because of cell maceration and autolysis, the likelihood of successful karyotype analysis of fetal tissue varies inversely with the time between fetal death and delivery. In an attempt to reduce the influence of these postmortem changes, we obtained amniotic fluid cells for cytogenetic studies from 12 fetuses as soon as possible after the diagnosis of fetal death was confirmed. We also obtained fetal tissue for cell culture in ten of the cases immediately following evacuation of the uterus. Cell culture was successful in 11 of 12 amniotic fluid specimens and in only one of ten fetal tissue specimens (p less than 0.001). Since the results of cytogenetic studies are of such importance in counseling patients regarding recurrence risk for fetal death, we recommend that amniotic fluid cells be obtained for karyotype analysis at the time of diagnosis of fetal death rather than awaiting delivery of a potentially macerated and autolyzed fetus.
染色体异常可能存在于约10%的胎儿死亡病例中。由于细胞浸软和自溶，对胎儿组织进行成功核型分析的可能性与胎儿死亡和分娩之间的时间成反比。为了减少这些死后变化的影响，在确诊胎儿死亡后，我们尽快从12例胎儿中获取羊水细胞用于细胞遗传学研究。在其中10例病例中，我们还在子宫排空后立即获取胎儿组织用于细胞培养。12份羊水标本中有11份细胞培养成功，而10份胎儿组织标本中只有1份成功（P<0.001）。由于细胞遗传学研究结果对于为患者提供胎儿死亡复发风险的咨询非常重要，我们建议在诊断胎儿死亡时获取羊水细胞进行核型分析，而不是等待可能已经浸软和自溶的胎儿分娩。
