Department of Virology and Parasitology, Fujita Health University School of Medicine, Dengakugakubo 1-98, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan.
Virus Res. 2010 Jan;147(1):77-84. doi: 10.1016/j.virusres.2009.10.009. Epub 2009 Oct 30.
Aichi virus (AiV), which is associated with acute gastroenteritis in humans, is a member of the genus Kobuvirus of the family Picornaviridae. Picornavirus genome replication occurs in replication complexes that include viral nonstructural proteins, host proteins and viral RNA. In poliovirus, all nonstructural proteins are found in the replication complexes, suggesting the ability of the viral nonstructural proteins to interact with each other. In this study, we examined the interactions between the AiV nonstructural proteins using a mammalian two-hybrid system. The results showed that all of the tested proteins could interact with more than one protein. We observed homodimerization of five proteins, bidirectional heterodimerization of six protein pairs, and unidirectional heterodimerization of eighteen protein pairs. Among the interactions detected in this study, the 2A-2BC, 2A-2BC, 2A-2C, 2BC-3CD, 2BC-3C, 2C-3C, 2C-3CD and 3AB-3C interactions have not been observed in the previous two-hybrid studies with other picornaviruses. The strongest interaction was observed between 2A and 3CD. AiV 2A has already been shown to be involved in genome replication. Domain mapping of the 2A and 3CD interaction in mammalian two-hybrid analysis revealed that the C-terminal quarter of 2A is not required for the interaction with 3CD.
爱知病毒（AiV）与人类急性肠胃炎有关，属于小核糖核酸病毒科肠道病毒属。小核糖核酸病毒的基因组复制发生在包含病毒非结构蛋白、宿主蛋白和病毒 RNA 的复制复合物中。在脊髓灰质炎病毒中，所有非结构蛋白都存在于复制复合物中，这表明病毒非结构蛋白之间有相互作用的能力。在这项研究中，我们使用哺乳动物双杂交系统来研究 AiV 非结构蛋白之间的相互作用。结果表明，所有测试的蛋白都可以与一种以上的蛋白相互作用。我们观察到五种蛋白的同源二聚体、六种蛋白对的双向异源二聚体和十八种蛋白对的单向异源二聚体。在本研究中检测到的相互作用中，2A-2BC、2A-2BC、2A-2C、2BC-3CD、2BC-3C、2C-3C、2C-3CD 和 3AB-3C 相互作用在以前使用其他小核糖核酸病毒的双杂交研究中没有观察到。最强的相互作用发生在 2A 和 3CD 之间。AiV 2A 已经被证明参与了基因组复制。哺乳动物双杂交分析中 2A 和 3CD 相互作用的结构域映射显示，2A 的 C 端四分之一不需要与 3CD 相互作用。
