Safra Children Hospital, Sheba Medical Center, Tel-Hashomer, Israel.
Lupus. 2009 Nov;18(13):1129-35. doi: 10.1177/0961203309345729.
Infections can act as environmental triggers that induce or promote systemic lupus erythematosus (SLE) in genetically predisposed individuals. New technologies, developed recently, enable simultaneous assessment of multiple antibodies. Antibodies to specific infectious agents may shed light into the mechanisms of induction of SLE. The aim of this study was to investigate the prevalence of seropositivity and the titers of antibodies to bacterial, viral, and parasitic agents in SLE patients compared with non-autoimmune controls. Sera from 260 individuals (120 SLE patients and 140 controls) were tested by the BioPlex 2200 Multiplexed Immunoassay method (BioRad) for the prevalence and titers of antibodies to eight infectious agents (Epstein-Barr virus: early antigen IgG, nuclear antigen IgG, viral capsid antigen IgG and IgM, heterophile IgM; cytomegalovirus IgG and IgM; Toxoplasma gondii IgG and IgM; rubella IgG and IgM; Treponema pallidum TPr15G, TPr17G, TPr47G; herpes simplex virus type 1 and 2 IgG; hepatitis C virus and hepatitis B core antibodies. Cytomegalovirus IgM and Epstein-Barr virus early antigen IgG (but not other Epstein-Barr virus antigens) were significantly more prevalent in SLE patients than in controls. Conversely, positive titers of hepatitis B core and rubella IgG antibodies were less prevalent in the SLE patients than in controls. Other differences in titer positivity prevalence were not detected between patients and controls. The titers of the cytomegalovirus IgM, Toxoplasma IgG, Epstein-Barr virus early antigen, and viral capsid antigen IgG antibodies were significantly higher in SLE compared with controls. Our data suggest the importance of previous exposure to infectious agents in the induction and the prevention of SLE.
感染可以作为环境触发因素，在遗传易感个体中诱导或促进系统性红斑狼疮（SLE）。最近开发的新技术使我们能够同时评估多种抗体。针对特定感染因子的抗体可以揭示 SLE 诱导的机制。本研究旨在调查与非自身免疫对照相比，SLE 患者中针对细菌、病毒和寄生虫病原体的血清阳性率和抗体滴度。通过 BioPlex 2200 多重免疫分析方法（BioRad），对 260 个人的血清（120 例 SLE 患者和 140 例对照）进行了检测，以确定针对八种感染因子（EB 病毒：早期抗原 IgG、核抗原 IgG、病毒衣壳抗原 IgG 和 IgM、异嗜性 IgM；巨细胞病毒 IgG 和 IgM；弓形虫 IgG 和 IgM；风疹 IgG 和 IgM；梅毒螺旋体 TPr15G、TPr17G、TPr47G；单纯疱疹病毒 1 和 2 IgG；丙型肝炎病毒和乙型肝炎核心抗体）的血清阳性率和抗体滴度。与对照组相比，SLE 患者中巨细胞病毒 IgM 和 EB 病毒早期抗原 IgG（而非其他 EB 病毒抗原）的阳性率明显更高。相反，SLE 患者中乙型肝炎核心和风疹 IgG 抗体的阳性滴度比对照组低。患者和对照组之间未检测到其他抗体滴度阳性率的差异。与对照组相比，SLE 患者的巨细胞病毒 IgM、弓形虫 IgG、EB 病毒早期抗原和病毒衣壳抗原 IgG 抗体的滴度显著更高。我们的数据表明，先前接触感染因子在 SLE 的诱导和预防中具有重要意义。
