Barker E, Mueller B M, Handgretinger R, Herter M, Yu A L, Reisfeld R A
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cancer Res. 1991 Jan 1;51(1):144-9.
An anti-GD2 ganglioside human/mouse chimeric monoclonal antibody, ch14.18, like its murine counterpart, 14.G2a, was shown to bind to human neuroblastoma cells. This chimeric antibody proved to be more effective than 14.G2a in mediating the lysis of neuroblastoma cells with human effector cells, such as granulocytes and natural killer cells within the peripheral blood mononuclear cell population. A comparison of these two effector cell populations isolated from the same donor revealed granulocytes to be more effective than peripheral blood mononuclear cells in lysing neuroblastoma cells, which were coated with monoclonal antibody ch14.18. Addition of recombinant human granulocyte-macrophage colony-stimulatory factor increased ch14.18-mediated lysis of neuroblastoma cells by granulocytes but not by peripheral blood mononuclear cells. In fact, granulocytes were effective in mediating lysis of neuroblastoma cells coated with ch14.18 irrespective of whether they were obtained from normal adults or from neuroblastoma patients.
一种抗GD2神经节苷脂人/鼠嵌合单克隆抗体ch14.18，与其鼠源对应物14.G2a一样，被证明可与人神经母细胞瘤细胞结合。在介导用人效应细胞（如外周血单个核细胞群体中的粒细胞和自然杀伤细胞）裂解神经母细胞瘤细胞方面，这种嵌合抗体被证明比14.G2a更有效。对从同一供体分离的这两种效应细胞群体进行比较发现，在裂解包被有单克隆抗体ch14.18的神经母细胞瘤细胞方面，粒细胞比外周血单个核细胞更有效。添加重组人粒细胞-巨噬细胞集落刺激因子可增加ch14.18介导的粒细胞对神经母细胞瘤细胞的裂解作用，但对外周血单个核细胞无此作用。事实上，无论粒细胞是从正常成年人还是从神经母细胞瘤患者获得，它们在介导裂解包被有ch14.18的神经母细胞瘤细胞方面都很有效。
