癌症治疗中新型治疗药物的靶基因发现。

Target gene discovery for novel therapeutic agents in cancer treatment.

作者信息

Ammerpohl Ole, Tiwari Sanjay, Kalthoff Holger

机构信息

Clinic for General Surgery and Thoracic Surgery, Division Molecular Oncology, University Hospital of Schleswig, Kiel, Germany.

出版信息

Methods Mol Biol. 2010;576:427-45. doi: 10.1007/978-1-59745-545-9_22.

DOI:10.1007/978-1-59745-545-9_22

PMID:19882275

Abstract

Target identification of novel therapeutic drugs is pivotal for the establishment of (1) new anticancer regimens, (2) to control side effects of the drugs, and (3) to identify appropriate combinations with established drugs. Here, we describe several in vitro assays applicable to characterize different characteristics of tumor cells. Furthermore, we present a protocol for establishing a reporter gene system for in vivo imaging, allowing for the study of drug effects in small animal models.

摘要

新型治疗药物的靶点识别对于建立（1）新的抗癌方案、（2）控制药物副作用以及（3）确定与现有药物的合适联合用药至关重要。在此，我们描述了几种适用于表征肿瘤细胞不同特性的体外测定方法。此外，我们还介绍了一种用于建立体内成像报告基因系统的方案，该系统可用于在小动物模型中研究药物效果。