Elution of proteins from gels.

New protein biochemical technologies have been developed that allow one to learn more and more about a protein with less and less material (on the order of microg). Polyacrylamide gel electrophoresis, which was originally strictly an analytical method, has in some cases become a high-resolution preparative method. This chapter will focus on the elution of proteins from SDS gels, with an emphasis on recovering enzyme or biological activity of the resulting protein.