Alliance Protein Laboratories, Thousand Oaks, California, USA.
J Pharm Sci. 2010 Apr;99(4):1674-92. doi: 10.1002/jps.21974.
Size exclusion chromatography (SEC) is the most widely used method for aggregation analysis of pharmaceutical proteins. However SEC analysis has a number of limitations, and one of the most important ones is protein adsorption to the resin. This problem is particularly severe when using new columns, and often column preconditioning protocols are required. This review focuses on the role that addition of various cosolvents to the mobile phase plays in suppressing that protein adsorption. Cosolvents such as salt, amino acids, and organic solvents are often used for this purpose. Because the protein interaction with the resin surface is highly heterogeneous, different cosolvents affect the protein adsorption differently. We will summarize the various effects of cosolvents on protein adsorption and retention and describe the mechanism of the cosolvent effects.
体积排阻色谱法（SEC）是最常用于药物蛋白聚集分析的方法。然而，SEC 分析存在一些局限性，其中最重要的问题之一是蛋白质对树脂的吸附。当使用新的柱子时，这个问题尤为严重，通常需要对柱子进行预处理。这篇综述重点讨论了在流动相中添加各种溶剂对抑制蛋白质吸附的作用。通常使用盐、氨基酸和有机溶剂等溶剂来达到这个目的。由于蛋白质与树脂表面的相互作用高度不均匀，不同的溶剂对蛋白质吸附的影响也不同。我们将总结溶剂对蛋白质吸附和保留的各种影响，并描述溶剂效应的机制。
