根癌农杆菌介导的几丁质酶和β-1,3-葡聚糖酶基因转化半夏
[Agrobacterium tumefaciens mediated Chitinase and beta-1,3-glucanase gene transformation for Pinellia ternata].
作者信息
Jin Bo, Jiang Fusheng, Yu Meirong, Chen Nipi, Ding Zhishan
机构信息
Life and science college of Zhejiang Chinese Medial University, Hangzhou 310053, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Jul;34(14):1765-7.
OBJECTIVE
To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.
METHOD
Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.
RESULT
PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.
CONCLUSION
The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata
目的
通过导入哈茨木霉的几丁质酶基因和β-1,3-葡聚糖酶基因，获得抗真菌的转基因半夏植株。
方法
以潮霉素磷酸转移酶作为选择标记，将几丁质酶基因（ech42）、β-1,3-葡聚糖酶基因（gluc78）以及由CaMV35S启动子驱动的双基因载体pCAMBIA(ech42 + gluc78) 通过农杆菌介导转化法导入半夏愈伤组织。
结果
PCR结果证实再生植株为转基因株系，RT-PCR结果证实外源基因构建体已转录为mRNA。根据株系的PCR结果，两个外源基因稳定遗传至T5代。
结论
结果表明几丁质酶基因ech42和β-1,3-葡聚糖酶基因gluc78分别或共同导入并整合到半夏中
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