Yao Jianhong, Pang Yongzhen, Qi Huaxiong, Wan Bingliang, Zhao Xiuyun, Kong Weiwen, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University, Shanghai 200433, PR China.
Transgenic Res. 2003 Dec;12(6):715-22. doi: 10.1023/b:trag.0000005146.05655.7d.
Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that the pta gene had integrated into the plant genome and northern blot analysis revealed transgene expression at various levels in transgenic plants. Genetic analysis confirmed Mendelian segregation of the transgene in T1 progeny. Insect bioassays showed that transgenic plants expressing PTA inhibited significantly the growth of peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic plants expressing pta confer enhanced resistance to aphids. Our study indicates that the pta gene can be used as a supplement to the snowdrop (Galanthus nivalis) lectin gene (gna) in the control of aphids, a sap-sucking insect pest causing significant yield losses of crops.
通过根癌农杆菌介导的转化方法，用含有选择标记新霉素磷酸转移酶基因（nptII）和半夏凝集素基因（pta）的质粒pBIPTA转化烟草叶片圆盘。再生出了32株独立的转基因烟草植株。PCR和Southern杂交分析证实pta基因已整合到植物基因组中，Northern杂交分析显示转基因在转基因植株中存在不同水平的表达。遗传分析证实了转基因在T1代子代中的孟德尔分离。昆虫生物测定表明，表达PTA的转基因植株显著抑制了桃蚜（Myzus persicae Sulzer）的生长。这是关于表达pta的转基因植株赋予增强的蚜虫抗性的首次报道。我们的研究表明，pta基因可作为雪花莲（Galanthus nivalis）凝集素基因（gna）的补充，用于防治蚜虫，蚜虫是一种吸食汁液的害虫，会导致作物显著减产。
