Zhang Ziqiang, Liu Yumei, Xue Bangqun, Wei Lan
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(15):1955-8.
To study the protective effects of catalpol against H2O2-induced oxidative damage in astrocytes.
Astrocytes were pretreated with 1, 10, 100 micromol x L(-1) catalpol and then exposed to H2O2. Effects of catalpol on cell morphous were observed by inverted microscope. Cell viability were detected by MTT assay. LDH content in the cell culture fluid, and intracellular T-SOD, GSH-Px activity and MDA content were assessed by colorimetric method.
The results showed that catalpol evidently ameliorated cell morphous, improved cell viability, and maintained integrality of cell membrane. Furthermore, catalpol could significantly inhibit the decrease of intracellullar T-SOD and GSH-Px activity, and the increase of MDA content.
Catalpol have protective effects against H2O2-induced oxidative damage in astrocytes.
研究梓醇对过氧化氢诱导的星形胶质细胞氧化损伤的保护作用。
用1、10、100微摩尔/升梓醇预处理星形胶质细胞，然后使其暴露于过氧化氢中。通过倒置显微镜观察梓醇对细胞形态的影响。采用MTT法检测细胞活力。用比色法评估细胞培养液中乳酸脱氢酶（LDH）含量以及细胞内总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）含量。
结果表明，梓醇明显改善细胞形态，提高细胞活力，并维持细胞膜的完整性。此外，梓醇可显著抑制细胞内T-SOD和GSH-Px活性的降低以及MDA含量的增加。
梓醇对过氧化氢诱导的星形胶质细胞氧化损伤具有保护作用。
Zotero 插件