Xue H, McCarron D A, Bukoski R D
Department of Medicine, Oregon Health Sciences University, Portland 97201.
Biochem Biophys Res Commun. 1991 Jan 15;174(1):11-7. doi: 10.1016/0006-291x(91)90477-o.
Contractile properties of rat mesenteric resistance arteries were studied immediately after isolation or after 48-hr incubation in culture medium [Dulbecco's modified Eagle's/Ham's F-12 (1:1) with insulin, transferrin and antibiotics]. Incubation in culture medium depressed active stress generating capacity, increased sensitivity to norepinephrine and ablated endothelium-dependent relaxation. The decrease in stress generation results from the loss of a releasable pool of intracellular Ca2+; the enhanced sensitivity is associated with decreased neuronal amine pump function. Addition of 300 pg/ml 1,25 (OH)2 vitamin D3 to the culture medium afforded nearly complete protection against the loss of stress generating capacity and partially preserved endothelial function. It is concluded that 1,25 (OH)2 vitamin D3 partially prevents phenotypic modulation of the vascular myocyte induced by culture conditions.
研究了大鼠肠系膜阻力动脉在分离后或在培养基[含有胰岛素、转铁蛋白和抗生素的杜氏改良伊格尔氏/哈姆氏F-12（1:1）]中培养48小时后的收缩特性。在培养基中培养会降低主动应激产生能力，增加对去甲肾上腺素的敏感性，并消除内皮依赖性舒张。应激产生的减少是由于细胞内可释放的钙离子池的丧失；敏感性增强与神经元胺泵功能降低有关。向培养基中添加300 pg/ml的1,25（OH）2维生素D3几乎完全防止了应激产生能力的丧失，并部分保留了内皮功能。得出的结论是，1,25（OH）2维生素D3部分预防了培养条件诱导的血管平滑肌细胞的表型调节。
