通过可变剪接产生两个相差一个密码子的mRNA可能是人类神经内分泌蛋白7B2基因表达的一种内在特性。

The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man.

作者信息

Paquet L, Lazure C, Seidah N G, Chrétien M, Mbikay M

机构信息

Laboratoires J.A. de Sève de Neuroendocrinologie Moléculaire et Biochimique, Institut de Recherches Cliniques de Montréal, Québec, Canada.

出版信息

Biochem Biophys Res Commun. 1991 Jan 15;174(1):156-62. doi: 10.1016/0006-291x(91)90499-w.

DOI:10.1016/0006-291x(91)90499-w

PMID:1989596

Abstract

Two types of mRNAs for neuroendocrine protein 7B2 were deduced from the sequence of cDNAs clones isolated from a human pituitary cDNA library. One type lacks an Ala100 codon present in the other. The difference is located at an intron site within the human 7B2 gene and can be explained by the transcriptional utilization of two alternate acceptor splice sites, three nucleotides apart. Heteroduplex analysis of DNA fragments amplified by the polymerase chain reaction indicated that this 7B2 mRNA dimorphism occurs in several human endocrine tissues as well as in other species, suggesting that the alternate processing of 7B2 gene transcripts may be an intrinsic mechanism of its expression and could underlie some yet unknown biological functions.

摘要

从人垂体cDNA文库中分离出的cDNA克隆序列推导得出神经内分泌蛋白7B2的两种mRNA类型。一种类型缺少另一种中存在的丙氨酸100密码子。这种差异位于人7B2基因内的一个内含子位点，可通过两个相距三个核苷酸的交替剪接受体位点的转录利用来解释。聚合酶链反应扩增的DNA片段的异源双链分析表明，这种7B2 mRNA二态性存在于几种人类内分泌组织以及其他物种中，这表明7B2基因转录本的交替加工可能是其表达的内在机制，并且可能是某些未知生物学功能的基础。