Agricultural Research Council, Research Centre for Meat Production and Genetic Improvement, Via Salaria, 31 00016 Monterotondo, Rome, Italy.
J Anim Sci. 2010 Feb;88(2):593-8. doi: 10.2527/jas.2009-2193. Epub 2009 Nov 6.
The purpose of this work was to produce and compare liver proteomic maps of cattle breeds that have been selected for different purposes: dairy (Holstein) and beef (Chianina). Liver was chosen because it displays functions of synthesis, homeostasis, excretion, and defense. Two dimensional electrophoresis analyses were performed on bovine livers collected from 4 individuals of each breed to evaluate differential protein expression. We found 9 spots differing in photodensity by >or=2-fold between the 2 breeds. Spots were analyzed through matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). A search against protein sequences in the National Center for Biotechnology Information databases indicated that the differentially expressed proteins correspond to liver-specific enzymes, with a major role in the metabolism of fatty acids, carbohydrates, AA, and the urea cycle.
本研究旨在生成并比较为不同目的选育的牛品种（乳用型荷斯坦牛和肉用型契安尼娜牛）的肝脏蛋白质组图谱。肝脏之所以被选中，是因为它具有合成、稳态、排泄和防御等多种功能。我们从每个品种的 4 头个体中采集肝脏，进行二维电泳分析，以评估差异蛋白的表达情况。结果发现，这两个品种之间有 9 个斑点的光密度差异超过 2 倍。利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和液相色谱串联质谱（LC-MS/MS）对斑点进行分析。与美国国家生物技术信息中心数据库中的蛋白质序列进行搜索表明，差异表达的蛋白与肝脏特异性酶相对应，在脂肪酸、碳水化合物、AA 和尿素循环的代谢中起主要作用。
