Department of Microbiology, China Agricultural University, Beijing, China.
Can J Microbiol. 2009 Sep;55(9):1070-5. doi: 10.1139/w09-064.
Spinosad is a new class of insecticides produced by Saccharopolyspora spinosa. The aim of this study was to construct a starch-utilizing strain that overproduced spinosad by intergeneric fusion between S. spinosa and Streptomyces avermitilis. Protoplast fusion is an important technique for engineering microbial strains, especially for microorganisms with few available molecular genetic tools. Protoplast fusion was conducted with UV-irradiated protoplasts of S. spinosa and S. avermitilis. Among 76 recombinants screened by ESI-MS and HPLC, a starch-utilizing strain F17, identified as S. spinosa, was obtained. The yield of spinosad in F17 was increased by 447.22%, compared with the yield of the wild-type strain. This is the first report of intergeneric protoplast fusion between S. spinosa and S. avermilitis, which shows great potential for industrial applications.
螺旋霉素是一种由棘孢菌（Saccharopolyspora spinosa）产生的新型杀虫剂。本研究旨在通过棘孢菌（Saccharopolyspora spinosa）和阿维链霉菌（Streptomyces avermitilis）的种间融合构建一种能够过量生产螺旋霉素的利用淀粉的菌株。原生质体融合是工程微生物菌株的重要技术，特别是对于分子遗传工具较少的微生物。用紫外辐射的棘孢菌和阿维链霉菌原生质体进行原生质体融合。在通过 ESI-MS 和 HPLC 筛选的 76 个重组体中，获得了一株被鉴定为棘孢菌的利用淀粉的菌株 F17。与野生型菌株相比，F17 中螺旋霉素的产量增加了 447.22%。这是首次报道棘孢菌和阿维链霉菌之间的种间原生质体融合，这表明其在工业应用方面具有巨大潜力。
