North-East Institute of Science and Technology, Council of Scientific and Industrial Research, Jorhat, Assam, India.
Can J Microbiol. 2009 Sep;55(9):1096-102. doi: 10.1139/w09-068.
A fungal strain was selected from the microbial repository of the North-East Institute of Science and Technology, Jorhat, India, which could produce a high yield of L-amino acid oxidase. 18SrRNA, ITS1, 5.8SrRNA ITS2, and partial 28 S rRNA sequencing and phenotypic characteristics indicate that it belong to the species Aspergillus fumigatus (designated as P13). Maximum production of enzyme (59.55 x 10-3 U/mg dry cell mass) was obtained in a medium containing 10 g/L glucose, 4 g/L yeast extract, and 4 g/L ammonium sulfate, with 20 mmol/L of L-threonine as the inducer. The optimum temperature for enzyme production was 30 degrees C at pH 7.0, with a shaking speed of 200 r/min. At 96 h, the enzyme activity was maximum. The A. fumigatus P13 L-amino acid oxidase accepts a broad substrate range, and the maximum enzyme activity (20.41 x 10-3 U/mg dry cell mass) was obtained with 50 mmol/L of L-tyrosine. In the literature, no reports have been found regarding the production of L-amino acid oxidase by A. fumigatus. The enzyme showed enantiomerically pure amino acid formation, which has tremendous demand in industrial applications.
一种真菌菌株从印度东北科技研究所的微生物库中被筛选出来，该菌株能够产生高产量的 L-氨基酸氧化酶。18SrRNA、ITS1、5.8SrRNA ITS2 和部分 28S rRNA 测序和表型特征表明，它属于烟曲霉（命名为 P13）。在含有 10g/L 葡萄糖、4g/L 酵母提取物和 4g/L 硫酸铵的培养基中，以 20mmol/L L-苏氨酸作为诱导剂，可获得最大酶产量（59.55×10-3U/mg 干细胞质量）。产酶的最适温度为 30°C，pH7.0，摇床转速为 200r/min。在 96h 时，酶活性达到最大值。A. fumigatus P13 L-氨基酸氧化酶接受广泛的底物范围，当使用 50mmol/L 的 L-酪氨酸时，可获得最大的酶活（20.41×10-3U/mg 干细胞质量）。在文献中，没有关于烟曲霉生产 L-氨基酸氧化酶的报道。该酶表现出对映体纯氨基酸形成，在工业应用中具有巨大的需求。
