Navarro S, Mitjavila M T, Katz A, Doly J, Vainchenker W
CNRS UPR 37, IRSC, Villejuif, France.
Exp Hematol. 1991 Jan;19(1):11-7.
Different human hematopoietic cell lines were analyzed for the presence of interleukin 6 (IL-6) and IL-6 receptor (IL-6-R). Both IL-6 mRNA and secreted active IL-6 protein were detectable in untreated cell lines with erythroid or megakaryoblastic features (K562, HEL, KU 812, MEG-01, and Dami), but they were not expressed constitutively in other leukemic cell lines (KG1, HL60, and U937). IL-6-R production, studied by the presence of its mRNA and specific binding sites for iodinated recombinant IL-6, was detected in most cell lines except K562, HEL, and Dami. Therefore, only KU 812 and MEG-01 coexpress both IL-6 and IL-6-R. After phorbol ester myristate acetate (PMA) treatment, all the cell lines studied expressed or overexpressed IL-6. In the erythroid K562 cell line, IL-6-R was not detectable before induction, but was promptly expressed after stimulation with PMA, suggesting that some of the new features of K562 cells induced by PMA may be mediated by IL-6. However, neutralizing antibodies against IL-6 did not block either the growth arrest or the loss of the erythroid phenotype induced by PMA. The presence of IL-6 and IL-6-R in erythroid and megakaryocytic leukemic cell lines suggests that their synthesis may occur during normal hematopoietic differentiation.
对不同的人类造血细胞系进行了白细胞介素6（IL-6）和IL-6受体（IL-6-R）的检测。在具有红系或巨核母细胞特征的未处理细胞系（K562、HEL、KU 812、MEG-01和Dami）中可检测到IL-6 mRNA和分泌的活性IL-6蛋白，但在其他白血病细胞系（KG1、HL60和U937）中它们不是组成性表达。通过其mRNA的存在和碘化重组IL-6的特异性结合位点研究IL-6-R的产生，在除K562、HEL和Dami之外的大多数细胞系中检测到。因此，只有KU 812和MEG-01共表达IL-6和IL-6-R。用佛波酯肉豆蔻酸乙酸酯（PMA）处理后，所有研究的细胞系均表达或过表达IL-6。在红系K562细胞系中，诱导前检测不到IL-6-R，但用PMA刺激后迅速表达，这表明PMA诱导的K562细胞的一些新特征可能由IL-6介导。然而，抗IL-6的中和抗体既不阻断PMA诱导的生长停滞也不阻断红系表型的丧失。红系和巨核细胞白血病细胞系中IL-6和IL-6-R的存在表明它们的合成可能发生在正常造血分化过程中。
